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Anti-PEDF 抗体 (ab14993)

Reviews (1)Q&A (3)References (9)
Western blot - Anti-PEDF antibody (ab14993)
  • Western blot - Anti-PEDF antibody (ab14993)

Key features and details

  • Rabbit polyclonal to PEDF
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-PEDF antibody [EPR9826(B)] (ab157207)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-PEDF antibody
    PEDF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to PEDF

  • 由来種

    Rabbit

  • 特異性

    Recognizes PEDF at 50kDa. Additional bands were detected at 66 and 76kDa, which were competed away by immunogen. These bands may be post translationally modified forms.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein (His-tag) corresponding to Human PEDF. Expressed in baby hamster kidney cells.
    Database link: P36955

  • ポジティブ･コントロール

    • WB: RIPA lysates from Y79 cells.

  • 特記事項

    For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab14993の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
特記事項
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

ターゲット情報

  • 機能

    Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

  • 組織特異性

    Retinal pigment epithelial cells and blood plasma.

  • 配列類似性

    Belongs to the serpin family.

  • 発生段階

    Expressed in quiescent cells.

  • ドメイン

    The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.

  • 翻訳後修飾

    The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

  • 細胞内局在

    Secreted. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cell proliferation-inducing gene 35 protein antibody
      • EPC 1 antibody
      • EPC-1 antibody
      • EPC1 antibody
      • OI12 antibody
      • OI6 antibody
      • PEDF antibody
      • PEDF_HUMAN antibody
      • PIG 35 antibody
      • PIG35 antibody
      • Pigment epithelium derived factor antibody
      • Pigment epithelium-derived factor antibody
      • Proliferation inducing protein 35 antibody
      • Serine (or cysteine) proteinase inhibitor antibody
      • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
      • Serpin F1 antibody
      • Serpin family F member 1 antibody
      • Serpin peptidase inhibitor antibody
      • Serpin peptidase inhibitor clade F member 1 antibody
      • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
      • SERPINF 1 antibody
      • Serpinf1 antibody
      see all

    画像

    • Western blot - Anti-PEDF antibody (ab14993)
      Western blot - Anti-PEDF antibody (ab14993)Kindly submitted by Dr John Wardale.

      Lanes 1-4: Cartilage lysates from 4 osteoarthritic knee replacement donors (100 mg (wet weight) powdered cartilage/ 1 ml RIPA buffer, extracted for 24 hrs at 4°C)

      Lane C: Positive control (overexpressing cell line)

      Anticipated band at approx 50kDa detected. Specificity of antibody allowed relatively long exposure and quite high concentrations of primary and secondary antibodies to be used with negligible background. Good results were also obtained with unreduced samples.
      The additional approx 250kDa band is likely to be due to PEDF crosslinked or bound to high molecular weight matrix proteins (collagen, aggrecan) which are present in cartilage lysates. Reduction using beta mercaptoethanol reduced some, but not all high MW staining and increased the intensity of the 50kDa band.

    • Western blot - Anti-PEDF antibody (ab14993)
      Western blot - Anti-PEDF antibody (ab14993)
      Anti-PEDF antibody (ab14993) at 1 µg/ml + Y79 (Human retinoblastoma cell line) cell lysate in RIPA buffer at 40 µg

      Secondary
      IgG (HRP) goat anti-rabbit

      Predicted band size: 50 kDa



      Chemiluminescence detection system.

    参考文献 (9)

    ab14993 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab14993 は 9 報の論文で使用されています。

    • Sun X  et al. Improvement of the bladder perfusion curative effect through tight junction protein degradation induced by magnetic temperature-sensitive hydrogels. Front Bioeng Biotechnol 10:958072 (2022). PubMed: 35992356
    • Li B  et al. lncRNA GABPB1 intronic transcript 1 upregulates pigment epithelium-derived factor via miR-93 to suppress cell proliferation in hepatocellular carcinoma. Oncol Lett 21:260 (2021). PubMed: 33664823
    • Zhang X  et al. Pigment epithelium-derived factor facilitates NLRP3 inflammasome activation through downregulating cytidine monophosphate kinase 2: A potential treatment strategy for missed abortion. Int J Mol Med 45:1436-1446 (2020). PubMed: 32323732
    • Qian W  et al. Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis. Mol Med Rep 17:6515-6525 (2018). WB . PubMed: 29532868
    • Han Z  et al. Retinal angiogenesis in the Ins2(Akita) mouse model of diabetic retinopathy. Invest Ophthalmol Vis Sci 54:574-84 (2013). WB . PubMed: 23221078
    • Allen CL  et al. Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing. PLoS One 8:e78441 (2013). ICC/IF . PubMed: 24205233
    • Tripathy D  et al. Age-related decrease in cerebrovascular-derived neuroprotective proteins: effect of acetaminophen. Microvasc Res 84:278-85 (2012). ELISA ; Rat . PubMed: 22944728
    • Sanchez A  et al. Pigment epithelium-derived factor (PEDF) protects cortical neurons in vitro from oxidant injury by activation of extracellular signal-regulated kinase (ERK) 1/2 and induction of Bcl-2. Neurosci Res 72:1-8 (2012). ELISA . PubMed: 21946416
    • Simper D  et al. Comparative proteomics profiling reveals role of smooth muscle progenitors in extracellular matrix production. Arterioscler Thromb Vasc Biol 30:1325-32 (2010). WB ; Human . PubMed: 20431068

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-PEDF antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Cartilage)
    Loading amount
    25 µg
    Specification
    Cartilage
    Gel Running Conditions
    Reduced Denaturing (4-20% gradient gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. XXXXXX XXXXXX

    Verified customer

    投稿 Sep 24 2008

    Question

    Thanks very much for your answer. I’m interested in purchase both pairs of antibodies (PEDF ab14993, ab30788 and Somatostatin ab64053 andab115489). I’ll place the order as soon as you send me the discount code.

    Read More
    Answer




    I am very pleased to hear you would like to accept our offer and test ab14993, ab30788 ,ab64053 and ab115489 in sELISA. These codes will give you: 1 freeprimary antibody per codebefore the expiration date. To redeem this offer, please submit an Abreview for sELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question

    I’ll would like to by anti-PEDF and Somatostatin antibodies for sandwish elisa (capture and detection) do you have any recommendation?

    Thanks for your attention

    Read More
    Answer

    Thank you very much for your interest in PEDF and Somatostatin antibodies.

    To our knowledge,none of our antibodies have been tested in sELISA. However, I have picked out four antibodies, which I found most suitable for this purpose:

    PEDF

    https://www.abcam.com/PEDF-antibody-ab14993.html (or use the following: https://www.abcam.com/PEDF-antibody-ab14993.html).

    https://www.abcam.com/PEDF-antibody-1C4-ab115489.html (or use the following: https://www.abcam.com/PEDF-antibody-1C4-ab115489.html).

    Somatostatin

    https://www.abcam.com/Somatostatin-antibody-ab64053.html (or use the following: https://www.abcam.com/Somatostatin-antibody-ab64053.html).

    https://www.abcam.com/Somatostatin-antibody-M09204-ab30788.html (or use the following: https://www.abcam.com/Somatostatin-antibody-M09204-ab30788.html).



    I can offer a discount off a future purchase if you buyone or bothof these antibody couplesnow, test it insELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody per antibody ordered.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab14993, ab30788, ab64053 andab115489 in sELISA. I will then send a discount code. This code must be issued before purchasing ab14993, ab30788, ab64053 and ab115489 so please wait for my reply before ordering.

    2. Purchase ab14993, ab30788, ab64053 and ab115489 either by phone, fax, or online (www.abcam.com).

    3. Test it in sELISA.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab14993, ab30788, ab64053 and ab115489 turns out to be unsuitable for sELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Question

    looking for negative control
    send database links (UniProt, Human Protein Atlas)

    Read More
    Answer

    Thank you for contacting us.

    Here are the links to the databases that could help identify potential tissue or cell types that might not express the PEDF protein.

    UniProt: http://www.uniprot.org/uniprot/P36955
    Human Protein Atlas: http://www.proteinatlas.org/ENSG00000132386

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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    For licensing inquiries, please contact partnerships@abcam.com