PE/Cy5® Anti-CD45.1 抗体 [A20] (ab25349)
Key features and details
- PE/Cy5® Mouse monoclonal [A20] to CD45.1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
-
製品名
PE/Cy5® Anti-CD45.1 antibody [A20]
CD45.1 一次抗体 製品一覧 -
製品の詳細
PE/Cy5® Mouse monoclonal [A20] to CD45.1 -
由来種
Mouse -
標識
PE/Cy5®. Ex: 496nm, Em: 670nm -
特異性
The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD 45.1 allotype. -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse CD45.1. SJL mouse thymocytes and splenocytes
-
特記事項
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% Gelatin, 16% Sucrose
Stabilising agent. -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
A20 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
-
Alternative Versions
- PerCP/Cy5.5® Anti-CD45.1 antibody [A20] (ab210237)
- violetFluor™ 450 Anti-CD45.1 antibody [A20] (ab241939)
- FITC Anti-CD45.1 antibody [A20] (ab24917)
- Cy5 ® Anti-CD45.1 antibody [A20] (ab24982)
- Biotin Anti-CD45.1 antibody [A20] (ab25011)
- Anti-CD45.1 antibody [A20] (ab25078)
- APC Anti-CD45.1 antibody [A20] (ab25348)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25349の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
-
関連性
CD45 is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants and as allelic forms CD45.1/Ly5.1 and CD45.2/Ly5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies in which resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. -
細胞内局在
Type I membrane protein. -
参照データベース
- Entrez Gene: 19264 Mouse
- SwissProt: P06800 Mouse
-
別名
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
see all
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab25349 は 2 報の論文で使用されています。
- Mardiney M & Malech HL Enhanced engraftment of hematopoietic progenitor cells in mice treated with granulocyte colony-stimulating factor before low-dose irradiation: implications for gene therapy. Blood 87:4049-56 (1996). PubMed: 8639760
- Vallera DA et al. The role of host T cell subsets in bone marrow rejection directed to isolated major histocompatibility complex class I versus class II differences of bm1 and bm12 mutant mice. Transplantation 57:249-56 (1994). PubMed: 7906058