PE/Cy5® Anti-CD4 抗体 [GK1.5] (ab25476)
![Flow Cytometry - PE/Cy5® Anti-CD4 antibody [GK1.5] (ab25476)](https://www.abcam.co.jp/ps/products/25/ab25476/Images/ab25476-276871-anti-cd4-antibody-gk15-pe-cy5-flow-cytometry.png "Flow Cytometry - PE/Cy5® Anti-CD4 antibody [GK1.5] (ab25476)")
Key features and details
- PE/Cy5® Rat monoclonal [GK1.5] to CD4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
PE/Cy5® Anti-CD4 antibody [GK1.5]
CD4 一次抗体 製品一覧 -
製品の詳細
PE/Cy5® Rat monoclonal [GK1.5] to CD4 -
由来種
Rat -
標識
PE/Cy5®. Ex: 496nm, Em: 670nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue/ cell preparation (Mouse) - cloned mouse CTL line V4.
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特記事項
This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.16% Sodium phosphate, 0.87% Sodium chloride
Also contains a stabilizing agent. -
Concentration information loading... -
精製度
Affinity purified -
一次抗体 備考
This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines. -
ポリ/モノ
モノクローナル -
クローン名
GK1.5 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25476の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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機能
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
配列類似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
細胞内局在
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 12504 Mouse
- SwissProt: P06332 Mouse
- Unigene: 2209 Mouse
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別名
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
画像
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Flow Cytometry - PE/Cy5® Anti-CD4 antibody [GK1.5] (ab25476)
Flow cytometry analysis showing C57Bl/6 splenocytes stained with ab25476 (sold line). The isotype control was PE-Cy5 Rat IgG2b.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab25476 は論文での使用が確認できていません。