PE/Cy5® Anti-CD3 epsilon 抗体 [C363.29B] (ab25531)
Key features and details
- PE/Cy5® Rat monoclonal [C363.29B] to CD3 epsilon
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG2c
製品の概要
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製品名
PE/Cy5® Anti-CD3 epsilon antibody [C363.29B]
CD3 epsilon 一次抗体 製品一覧 -
製品の詳細
PE/Cy5® Rat monoclonal [C363.29B] to CD3 epsilon -
由来種
Rat -
標識
PE/Cy5®. Ex: 496nm, Em: 670nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Full length protein corresponding to Mouse CD3 epsilon. " IL-4 producing Th2 cell lines including D10"
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エピトープ
This antibody recognises an epitope on the 25kDa epsilon chain of the CD3/TCR complex. -
特記事項
In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% Gelatin, 16% Sucrose
Also contains a stabilizing agent. -
Concentration information loading...
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精製度
IgG fraction -
一次抗体 備考
In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining and is a good immunoprecipitating antibody. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided). -
ポリ/モノ
モノクローナル -
クローン名
C363.29B -
アイソタイプ
IgG2c -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25531の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use 1µg for 106 cells.
|
特記事項 |
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Flow Cyt
Use 1µg for 106 cells. |
ターゲット情報
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機能
The CD3 complex mediates signal transduction. -
配列類似性
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12501 Mouse
- SwissProt: P22646 Mouse
- Unigene: 210361 Mouse
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別名
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab25531 は 1 報の論文で使用されています。
- Carrithers MD et al. Differential adhesion molecule requirements for immune surveillance and inflammatory recruitment. Brain 123 ( Pt 6):1092-101 (2000). PubMed: 10825349