Anti-Pea3 抗体 [OTI5C11] (ab236503)
![Western blot - Anti-Pea3 antibody [OTI5C11] (ab236503)](https://www.abcam.co.jp/ps/products/236/ab236503/Images/ab236503-312858-03.jpg "Western blot - Anti-Pea3 antibody [OTI5C11] (ab236503)")
Key features and details
- Mouse monoclonal [OTI5C11] to Pea3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Pea3 antibody [OTI5C11]
Pea3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OTI5C11] to Pea3 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human Pea3 aa 1-276. (NP_001073143) produced in E.coli.
Database link: P43268 -
ポジティブ･コントロール
- WB: HEK-293T transfected with pCMV6-ENTRY Pea3 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Purified from cell culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
OTI5C11 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236503の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 54 kDa.
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| 特記事項 |
|---|
|
WB
1/2000. Predicted molecular weight: 54 kDa. |
ターゲット情報
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機能
Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. -
配列類似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
翻訳後修飾
Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2118 Human
- Omim: 600711 Human
- SwissProt: P43268 Human
- Unigene: 434059 Human
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別名
- Adenovirus E1A enhancer binding protein antibody
- Adenovirus E1A enhancer-binding protein antibody
- E1A F antibody
see all
画像
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Western blot - Anti-Pea3 antibody [OTI5C11] (ab236503)All lanes : Anti-Pea3 antibody [OTI5C11] (ab236503) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY Pea3, cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 54 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab236503 は論文での使用が確認できていません。