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PE Anti-Vimentin 抗体 [VI-RE/1] (ab49918)

Submit a review Submit a question References (5)
Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
  • Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
  • Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

Key features and details

  • PE Mouse monoclonal [VI-RE/1] to Vimentin
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

APC Unconjugated

製品の概要

  • 製品名

    PE Anti-Vimentin antibody [VI-RE/1]
    Vimentin 一次抗体 製品一覧

  • 製品の詳細

    PE Mouse monoclonal [VI-RE/1] to Vimentin

  • 由来種

    Mouse

  • 標識

    PE. Ex: 488nm, Em: 575nm

  • 特異性

    The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human
    非交差種: Mouse, Pig

  • 免疫原

    Recombinant full length protein corresponding to Human Vimentin aa 1 to the C-terminus.
    Database link: P08670

  • ポジティブ･コントロール

    • ICC/IF: HeLa cells Flow cyt: LEP-19 cells.

  • 特記事項

    Conjugated with R-Phycoerythrin (PE) under optimum conditions.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab49918の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 10 µg/ml.
Flow Cyt (Intra)
Use a concentration of 1 - 5 µg/ml.
特記事項
ICC/IF
Use a concentration of 10 µg/ml.
Flow Cyt (Intra)
Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

  • 組織特異性

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

  • 関連疾患

    Cataract 30

  • 配列類似性

    Belongs to the intermediate filament family.

  • ドメイン

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

  • 翻訳後修飾

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 製品の状態

      Vimentin is found in connective tissue and in the cytoskeleton.

    • 別名

      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
      Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

      Separation of ESS-1 cells (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with ESS-1 cells stained using ab49918 at 0.56 μg/ml.

    • Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
      Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

      ab49918 staining Vimentinn in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab49918 at a working concentration of 10μg/ml (shown in red) and ab185031, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
      Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

      Flow cytometry analysis (intracellular staining) of Vimentin  in LEP-19 human fibroblast cell line using ab49918 anti-human Vimentin (VI-RE/1) PE. Overlay with Isotype mouse IgG1 control. 

    参考文献 (5)

    ab49918 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab49918 は 5 報の論文で使用されています。

    • Guo YX  et al. Diagnostic value of HE4+ circulating tumor cells in patients with suspicious ovarian cancer. Oncotarget 9:7522-7533 (2018). PubMed: 29484129
    • Cervelló I  et al. Reconstruction of endometrium from human endometrial side population cell lines. PLoS One 6:e21221 (2011). Flow Cyt ; Human . PubMed: 21712999
    • Basu D  et al. Detecting and targeting mesenchymal-like subpopulations within squamous cell carcinomas. Cell Cycle 10:2008-16 (2011). PubMed: 21558812
    • Basu D  et al. Evidence for mesenchymal-like sub-populations within squamous cell carcinomas possessing chemoresistance and phenotypic plasticity. Oncogene : (2010). Flow Cyt . PubMed: 20498638
    • Meyer MJ  et al. Dynamic regulation of CD24 and the invasive, CD44posCD24neg phenotype in breast cancer cell lines. Breast Cancer Res 11:R82 (2009). PubMed: 19906290

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