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AB25452

PE Anti-VCAM1 抗体 [M/K-2]

PE Anti-VCAM1 antibody [M/K-2]

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(1 Publication)

Rat Monoclonal VCAM1 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

別名を表示する

CD106, Vcam-1, Vcam1, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1

1 Images
Flow Cytometry - PE Anti-VCAM1 antibody [M/K-2] (AB25452)
  • Flow Cyt

Unknown

Flow Cytometry - PE Anti-VCAM1 antibody [M/K-2] (AB25452)

Flow cytometry analysis staining VCAM1 in mouse bone marrow cells using ab25452 at a dilution of 0.1 μg/106 cells.

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

M/K-2

アイソタイプ

IgG1

軽鎖のタイプ

kappa

標識

PE

励起波長/蛍光波長

Ex: 480;565nm, Em: 578nm

キャリアフリー

No

交差種

Mouse

アプリケーション

Flow Cyt

applications

特異性

ab25452 recognises CD106/VCAM1 (100 kDa)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p>ab154449 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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製品の詳細

The M/K-2 monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long term cardiac graft survival. It immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under nonreducing conditions.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
バッファー組成
pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.
See full target information Vcam1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Leukemia research 37:1374-82 PubMed23806234

2013

Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia.

Applications

Flow Cyt

Species

Mouse

C-H Olme,R Finnon,N Brown,S Kabacik,S D Bouffler,C Badie
View all publications
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代替製品

Primary Antibodies

AB223982

FITC Anti-VCAM1 antibody [EPR5047]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - FITC Anti-VCAM1 antibody [EPR5047] (AB223982)

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  • 0
Primary Antibodies

AB195540

HRP Anti-VCAM1 antibody [EPR5047]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-VCAM1 antibody [EPR5047] (AB195540)

  • 0
  • 0
Primary Antibodies

AB223981

PE Anti-VCAM1 antibody [EPR5047]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-VCAM1 antibody [EPR5047] (AB223981)

  • 0
  • 0
Primary Antibodies

AB215380

Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free (AB215380)

  • 0
  • 0
Primary Antibodies

AB194319

Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047] (AB194319)

  • 5
  • 1
Primary Antibodies

AB174279

Anti-VCAM1 antibody [EPR5038(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

  • 0
  • 0
Primary Antibodies

AB134047

Anti-VCAM1 antibody [EPR5047]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

  • 5
  • 16

組合せ製品

Assay Kits

AB201278

Mouse VCAM1 ELISA Kit (CD106)

Recombinant|SimpleStep

Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB226

FITC Anti-CD90 / Thy1 antibody [MRC OX-7]

Flow Cytometry (Intracellular) - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (AB226)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB282575

Anti-ICAM1 antibody [EPR24639-3]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR24639-3] (AB282575)

  • 5
  • 1

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