AB95532

PE Anti-TRAIL 抗体 [N2B2]

Name: PE Anti-TRAIL 抗体 [N2B2] (ab95532) | Abcam
Brand: Abcam Limited
SKU: AB95532
Price: 38000 JPY
Availability: InStock

PE Anti-TRAIL antibody [N2B2]

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(3 Publications)

Anti-TRAIL antibody [N2B2] conjugated to PE - suitable for flow cytometry, and reacts with mouse samples. Clone [N2B2] is a rat monoclonal antibody cited in 15 publications.

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別名を表示する

CD253, Trail, Tnfsf10, Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL

1 Images

Flow Cytometry - PE Anti-TRAIL antibody [N2B2] (AB95532)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-TRAIL antibody [N2B2] (AB95532)

Flow cytometry staining of TRAIL-transfected cells with 0.06 μg of Rat IgG2a Isotype Control PE (pink histogram) or 0.06 μg of ab95532 (green histogram). Total viable cells were used for analysis.

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

N2B2

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

標識

励起波長/蛍光波長

Ex: 480;565nm, Em: 578nm

キャリアフリー

交差種

Mouse

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing Tnfsf10 protein. The exact immunogen used to generate this antibody is proprietary information.

P50592

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.06-0.125 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>Staining the cell sample in a final volume of 100μl is recommended.</p><p><a href='/products/unavailable/rat-igg2a-kappa-monoclonal-klhg2a-1-1-pe-isotype-control-ab134674'>ab134674</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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製品の詳細

ab95532 inhibits IL-2 or IL-15 activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TRAIL also known as TNF-related apoptosis-inducing ligand plays an important role in inducing apoptosis. This protein has a mass of around 32 kDa and is expressed across various tissues including immune cells. TRAIL interacts with specific receptors on the cell surface to initiate a signaling cascade that results in programmed cell death. It is an attractive therapeutic target because of its selective ability to induce apoptosis in cancer cells while sparing most normal cells.

Biological function summary

TRAIL's function extends beyond inducing cell death. It is an important member of the tumor necrosis factor (TNF) superfamily which regulates immune responses and inflammation. TRAIL can form a homotrimeric complex allowing it to bind effectively to death receptors on target cells specifically DR4 and DR5 triggering a series of downstream signaling events. This interaction is significant in modulating immune surveillance and tumor suppression.

Pathways

TRAIL is integral to both intrinsic and extrinsic apoptosis pathways. Through these pathways TRAIL is closely related to FADD and caspase-8 which are important for apoptosis induction. The extrinsic pathway involves binding to TRAIL death receptors leading to DISC formation while the intrinsic pathway involves mitochondrial events mediated by members of the Bcl-2 protein family.

TRAIL has a significant connection with cancer and autoimmune diseases. TRAIL plays a dual role in cancer where it acts as a tumor suppressor but can also promote tumor progression if cancer cells become resistant to its effects. In cancer TRAIL’s interaction with proteins like FLIP can alter the apoptotic potential of the cells. In autoimmune diseases TRAIL's dysregulation can contribute to tissue damage due to increased immune activity highlighting its complex role in maintaining immune balance.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

See full target information Tnfsf10

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature medicine 12:214-9 PubMed16444265

2006

A novel dendritic cell subset involved in tumor immunosurveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Taieb,Nathalie Chaput,Cédric Ménard,Lionel Apetoh,Evelyn Ullrich,Mathieu Bonmort,Marie Péquignot,Noelia Casares,Magali Terme,Caroline Flament,Paule Opolon,Yann Lecluse,Didier Métivier,Elena Tomasello,Eric Vivier,François Ghiringhelli,François Martin,David Klatzmann,Thierry Poynard,Thomas Tursz,Graça Raposo,Hideo Yagita,Bernard Ryffel,Guido Kroemer,Laurence Zitvogel

Journal of immunology (Baltimore, Md. : 1950) 172:123-9 PubMed14688317

2003

NK cell TRAIL eliminates immature dendritic cells in vivo and limits dendritic cell vaccination efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihiro Hayakawa,Valentina Screpanti,Hideo Yagita,Alf Grandien,Hans-Gustaf Ljunggren,Mark J Smyth,Benedict J Chambers

Journal of immunology (Baltimore, Md. : 1950) 163:1906-13 PubMed10438925

1999

Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells.

Applications

Unspecified application

Species

Unspecified reactive species

N Kayagaki,N Yamaguchi,M Nakayama,K Takeda,H Akiba,H Tsutsui,H Okamura,K Nakanishi,K Okumura,H Yagita

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-TRAIL 抗体 [N2B2]

PE Anti-TRAIL antibody [N2B2]

Flow Cytometry - PE Anti-TRAIL antibody [N2B2] (AB95532)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise