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PE Anti-TLR3 抗体 [40C1285.6] (ab45093)

Reviews (2) Submit a question References (2)
Flow Cytometry (Intracellular) - PE Anti-TLR3 antibody [40C1285.6] (ab45093)
  • Flow Cytometry (Intracellular) - PE Anti-TLR3 antibody [40C1285.6] (ab45093)

Key features and details

  • PE Mouse monoclonal [40C1285.6] to TLR3
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

製品の概要

  • 製品名

    PE Anti-TLR3 antibody [40C1285.6]
    TLR3 一次抗体 製品一覧

  • 製品の詳細

    PE Mouse monoclonal [40C1285.6] to TLR3

  • 由来種

    Mouse

  • 標識

    PE. Ex: 488nm, Em: 575nm

  • アプリケーション

    適用あり: Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human

  • 免疫原

    This antibody was developed against KLH-conjugated synthetic peptide of human TLR3.

  • ポジティブ･コントロール

    • Human intestine or Ramos. Flow Cyt (Intra): Ramos, A549 cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab45093の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
特記事項
追加情報
FACS:(Intracellular) Use 2 - 5µg for 106 cells.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 104 kDa.

This antibody has tested negative by western blot in mouse intestine cell lysate.



Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

  • 機能

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

  • 組織特異性

    Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.

  • 関連疾患

    Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.

  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.

  • ドメイン

    ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.

  • 翻訳後修飾

    Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.

  • 細胞内局在

    Endoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD283 antibody
      • CD283 antigen antibody
      • IIAE2 antibody
      • TLR 3 antibody
      • Tlr3 antibody
      • TLR3_HUMAN antibody
      • Toll Like Receptor 3 antibody
      • Toll-like receptor 3 antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - PE Anti-TLR3 antibody [40C1285.6] (ab45093)
      Flow Cytometry (Intracellular) - PE Anti-TLR3 antibody [40C1285.6] (ab45093)This image is courtesy of an anonymous Abreview

      ab45093 staining TLR3 in Human A549 (lung epithelial) cell line by Flow Cytometry. The sample was incubated with the primary antibody (5μg/ml in 0.1% Saponin + 1% FCS in PBS) for 45 minutes at 4°C. 1 = isotype control, 2 = ab45093

       

      See Abreview

    • Flow Cytometry (Intracellular) - PE Anti-TLR3 antibody [40C1285.6] (ab45093)
      Flow Cytometry (Intracellular) - PE Anti-TLR3 antibody [40C1285.6] (ab45093)
      ab45093, at 3 ug/ 1X10^6 cells, staining Ramos cells by Intracellular flow cytometric analysis. Toll-Like-Receptor3 (open peak) and isotype negative control (shaded peak).

    参考文献 (2)

    ab45093 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab45093 は 2 報の論文で使用されています。

    • Tolstova T  et al. The effect of TLR3 priming conditions on MSC immunosuppressive properties. Stem Cell Res Ther 14:344 (2023). PubMed: 38031182
    • Franzen-Röhl E  et al. Increased cell-mediated immune responses in patients with recurrent herpes simplex virus type 2 meningitis. Clin Vaccine Immunol 18:655-60 (2011). Flow Cyt . PubMed: 21325490

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-TLR3 antibody [40C1285.6] (Phycoerythrin)

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (5-16% gels)
    Sample
    Human Tissue lysate - whole (see below)
    Specification
    see below
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 01 2013

    Flow Cytometry abreview for Anti-TLR3 antibody [40C1285.6] (Phycoerythrin)

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - 0,1% Saponin / PBS
    Sample
    Human Cell (A549 cells (lung epithelial cells))
    Specification
    A549 cells (lung epithelial cells)
    Gating Strategy
    no gate
    Preparation
    Cell harvesting/tissue preparation method: cell culture (commercially available cell line)
    Sample buffer: 0,1% Saponin /1% FCS/ PBS
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 23 2013

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