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AB223917

PE Anti-STAT6 抗体 [YE361]

PE Anti-STAT6 antibody [YE361]

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(1 Publication)

Rabbit Recombinant Monoclonal STAT6 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Mouse samples. Cited in 1 publication.

別名を表示する

Signal transducer and activator of transcription 6, IL-4 Stat, STAT6

1 Images
Flow Cytometry (Intracellular) - PE Anti-STAT6 antibody [YE361] (AB223917)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-STAT6 antibody [YE361] (AB223917)

Overlay histogram showing NIH3T3 cells stained with ab223917 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223917, 1/1000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in NIH3T3 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

関連する標識済み抗体及び組成の異なる製品 (10)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

YE361

アイソタイプ

IgG

標識

PE

励起波長/蛍光波長

Ex: 480;565nm, Em: 578nm

キャリアフリー

No

交差種

Mouse

アプリケーション

Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Do Not Freeze|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.
Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Carries out a dual function : signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
See full target information STAT6
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35806053

2022

Synthesis and Biological Evaluation of Small Molecules as Potential Anticancer Multitarget Agents.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Pla-López,Raquel Castillo,Rocío Cejudo-Marín,Olaya García-Pedrero,Mariam Bakir-Laso,Eva Falomir,Miguel Carda
View all publications
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代替製品

Primary Antibodies

AB32108

Anti-STAT6 antibody [E265]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-STAT6 antibody [E265] (AB32108)

  • 0
  • 0
Primary Antibodies

AB188080

Anti-STAT6 (phospho Y641) antibody [EPR18278-265]

RabMAb|Recombinant

Western blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

  • 0
  • 0
Primary Antibodies

AB217998

Anti-STAT6 antibody [EPR18278-222]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (AB217998)

  • 0
  • 0

組合せ製品

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB68153

Anti-STAT3 antibody [EPR787Y]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-STAT3 antibody [EPR787Y] (AB68153)

  • 4
  • 10
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Primary Antibodies

AB92506

Anti-STAT1 alpha antibody [EPYR2154]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Advanced Validation

Immunoprecipitation - Anti-STAT1 alpha antibody [EPYR2154] (AB92506)

  • 4
  • 2

Abcam product promise

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