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PE Anti-PSMA 抗体 [GCP-05] (ab77228)

Submit a review Q&A (7)References (8)
Flow Cytometry - PE Anti-PSMA antibody [GCP-05] (ab77228)

    Key features and details

    • PE Mouse monoclonal [GCP-05] to PSMA
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Unconjugated

    製品の概要

    • 製品名

      PE Anti-PSMA antibody [GCP-05]
      PSMA 一次抗体 製品一覧

    • 製品の詳細

      PE Mouse monoclonal [GCP-05] to PSMA

    • 由来種

      Mouse

    • 標識

      PE. Ex: 488nm, Em: 575nm

    • 特異性

      ab77228 recognizes extracellular domain of PSMA, expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker.

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Recombinant fragment, corresponding to amino acids 44-750 of Human PSMA

    • 特記事項

      The antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab77228の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use a concentration of 5 µg/ml.

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 
    特記事項
    Flow Cyt
    Use a concentration of 5 µg/ml.

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

    ターゲット情報

    • 機能

      Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
      Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

    • 組織特異性

      Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.

    • 配列類似性

      Belongs to the peptidase M28 family. M28B subfamily.

    • ドメイン

      The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.

    • 翻訳後修飾

      The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
      The N-terminus is blocked.

    • 細胞内局在

      Cytoplasm and Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Cell growth inhibiting protein 27 antibody
        • Cell growth-inhibiting gene 27 protein antibody
        • FGCP antibody
        • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
        • Folate hydrolase 1 antibody
        • Folate hydrolase antibody
        • Folate hydrolase prostate specific membrane antigen 1 antibody
        • FOLH 1 antibody
        • FOLH antibody
        • Folh1 antibody
        • FOLH1_HUMAN antibody
        • Folylpoly gamma glutamate carboxypeptidase antibody
        • Folylpoly-gamma-glutamate carboxypeptidase antibody
        • GCP 2 antibody
        • GCP II antibody
        • GCP2 antibody
        • GCPII antibody
        • GIG27 antibody
        • Glutamate carboxylase II antibody
        • Glutamate carboxypeptidase 2 antibody
        • Glutamate carboxypeptidase II antibody
        • Membrane glutamate carboxypeptidase antibody
        • mGCP antibody
        • N acetylated alpha linked acidic dipeptidase 1 antibody
        • N-acetylated-alpha-linked acidic dipeptidase I antibody
        • NAALAD 1 antibody
        • NAALAD1 antibody
        • NAALAdase antibody
        • NAALADase I antibody
        • Prostate specific membrane antigen antibody
        • Prostate specific membrane antigen variant F antibody
        • Prostate-specific membrane antigen antibody
        • PSM antibody
        • PSMA antibody
        • Pteroylpoly gamma glutamate carboxypeptidase antibody
        • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
        see all

      画像

      • Flow Cytometry - PE Anti-PSMA antibody [GCP-05] (ab77228)
        Flow Cytometry - PE Anti-PSMA antibody [GCP-05] (ab77228)

        Surface staining (flow cytometry) of GCPII / PSMA using anti-GCPII (GCP-05) PE on LNCaP cell line.

      参考文献 (8)

      ab77228 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab77228 は 8 報の論文で使用されています。

      • Glud EN  et al. Identification of a high-risk immunogenic prostate cancer patient subset as candidates for T-cell engager immunotherapy and the introduction of a novel albumin-fused anti-CD3 × anti-PSMA bispecific design. Br J Cancer 127:2186-2197 (2022). PubMed: 36243890
      • Papasotiriou I  et al. Study and detection of potential markers for predicting metastasis into lymph nodes in prostate cancer. Biomark Med 14:1317-1327 (2020). PubMed: 32799659
      • Yunger S  et al. Tumor-infiltrating lymphocytes from human prostate tumors reveal anti-tumor reactivity and potential for adoptive cell therapy. Oncoimmunology 8:e1672494 (2019). PubMed: 31741775
      • Minn I  et al. Imaging CAR T cell therapy with PSMA-targeted positron emission tomography. Sci Adv 5:eaaw5096 (2019). PubMed: 31281894
      • Sácha P  et al. Expression of glutamate carboxypeptidase II in human brain. Neuroscience 144:1361-72 (2007). PubMed: 17150306
      • Barinka C  et al. Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding. Eur J Biochem 271:2782-90 (2004). PubMed: 15206943
      • Barinka C  et al. Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity. Protein Sci 13:1627-35 (2004). PubMed: 15152093
      • Barinka C  et al. Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II. J Neurochem 80:477-87 (2002). PubMed: 11905994

      レビューと Q&A

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      レビューを送る 質問を送る

      1-7 of 7 Abreviews or Q&A

      Question

      How many molecules of PE are attached to antibody?

      Read More
      Answer

      I can confirm that there is only one Phycoerythrin molecule conjugated to one antibody molecule in this product (ab77228).

      Read More

      Question

      Please let me know of the protocol used to validate the GCP-05 clone (ab77228) with LnCaP cells. Also, I would appreciate any reference for the GCP-05 clone.

      Read More
      Answer



      For western blotting with GCP-05 with LnCaP cells, one just needs a standard whole cell extract. There is no LnCaP-focused procedure that I am aware of.

      Regarding references, all publications we are aware of that have used this antibody can be found under the 'References' tab on the online datasheet. Here is the link to those references: https://www.abcam.com/PSMA-antibody-GCP-05-Phycoerythrin-ab77228-references.html

      Read More

      Question

      WHat type of samples were tested by you? WHat is a good positive control?

      Read More
      Answer

      Thank you for contacting us. The testing by the laboratory was done using LNCaP cells, so these would be a good positive control as well as the cell types used in the references.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      Customer kindly contacted us to inquire if this product or other anti-PSMA antibodies in our catalog would be conformationally specific.

      Read More
      Answer

      Thank you for contacting us.

      After review of our catalog and discussion with the lab, I am sorry to inform you that our current anti-PSMA products appear to either be conformationally independent or not tested for conformational bias. While this search has not yielded the specific results we had hoped for, I would be very happy to help you to find products which may recognize other features or isoforms of PSMA.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      I am currently trying to identify the levels of PSMA expression in human cell and tumor lysates by western blot analysis in both reducing and native conditions and was wondering if you have a primary antibody that would be useful in both of these applications? I have been using your YPSMA antibody but have found differing expression levels for the same cells under reducing and native conditions and would like to optimize this assay as much as possible.

      Any information you can give would be greatly appreciated,

      Read More
      Answer

      Thank you for contacting us.

      We do not have anti PSMA antibody that would detect both the native as well as reduced form of protein. We however have more than 20 products that are used under reducing conditions and gave best results. Taking ion consideration the current data we have, It is hard to tell which antibody is suitable for native protein, theoretically every antibody should detect however there are certain antibodies that works well under denatured and reduced conditions rather than native.

      I am sorry we are not aware about our PSMA antibodies for their use under native conditions. This has to determined by the end user.

      I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      Hello, I'm contacting your technical services because I spend a month working with your GCP-04 anti PSMA antibody (ab66911) for FACS analysis on LNCaP and C4-2 cells (PSMA positives cells) without any signal. I tested the GCP-05-PE (ab77228) and I had the them result: no signal. Yesterday I tested fresh LNCaP cells and two other antibodies anti-PSMA (a gift from one of our collaborator) and I had significant results even on our "old" C4-2 cells (see attached file). As you are attached to the quality of your products, can you offer us a refund or a replacement. Regards

      Read More
      Answer

      Thank you for contacting us and for reporting the problem you experienced with the antibodies ab66911 and ab77228. I am sorry that the product you received did not perform as stated on the datasheet. We will use the data you provided to investigate the source of the problem with those products. As requested, I will be happy to issue you in compensation either free of charge replacements with ab76104 (tested in Human and flow cytometry, www.abcam.com/ab76104), a credit note or a refund against the original orders. I look forward to hearing from you with details of how you would like to proceed. Could you also please send me one of the following : the order number, the PO number, the date of purchase or at least the address of your lab? We apologize for the inconvenience this has caused.

      Read More

      Question

      What is the fluorochrome ration for this PE conjugated PSMA antibody?

      Read More
      Answer

      Thank you for your inquiry, and for your patience while we looked into this. The fluorochrome ratio for this antibody is 1:1. I hope this helps. Please do not hesitate to contact us if you need any additional assistanc

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