PE Anti-PKR 抗体 [EPR19374] (ab225322)
![Flow Cytometry (Intracellular) - PE Anti-PKR antibody [EPR19374] (ab225322)](https://www.abcam.co.jp/ps/products/225/ab225322/Images/ab225322-312720-anti-pkr-antibody-epr19374-phycoerythrin-flow-cytometry.jpg "Flow Cytometry (Intracellular) - PE Anti-PKR antibody [EPR19374] (ab225322)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR19374] to PKR
- Suitable for: Flow Cyt (Intra)
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
製品の概要
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製品名
PE Anti-PKR antibody [EPR19374]
PKR 一次抗体 製品一覧 -
製品の詳細
PE Rabbit monoclonal [EPR19374] to PKR -
由来種
Rabbit -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Human
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免疫原
Recombinant fragment within Mouse PKR aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q03963 -
ポジティブ･コントロール
- Flow Cyt (intra): NIH/3T3 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19374 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab225322の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/5000.
The cellular localisation of this product has been verified in ICC/IF. |
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/5000. The cellular localisation of this product has been verified in ICC/IF. |
ターゲット情報
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機能
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. - Information by UniProt
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参照データベース
- Entrez Gene: 5610 Human
- Entrez Gene: 19106 Mouse
- Entrez Gene: 54287 Rat
- Omim: 176871 Human
- SwissProt: P19525 Human
- SwissProt: Q03963 Mouse
- SwissProt: Q63184 Rat
- Unigene: 131431 Human
see all -
別名
- Double stranded RNA activated protein kinase; antibody
- E2AK2_HUMAN antibody
- eIF-2A protein kinase 2 antibody
see all
画像
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Flow Cytometry (Intracellular) - PE Anti-PKR antibody [EPR19374] (ab225322)
Overlay histogram showing NIH/3T3 cells stained with ab225322 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225322, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![PE Anti-PKR antibody [EPR19374] (ab225322)](https://www.abcam.co.jp/ps/products/225/ab225322/Images/ab225322-3-benefits-of-recombinant-antibodies.png)
PE Anti-PKR antibody [EPR19374] (ab225322)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab225322 は論文での使用が確認できていません。