PE Anti-pan Cytokeratin 抗体 [C-11] (ab52460)
![Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)](https://www.abcam.co.jp/ps/products/52/ab52460/Images/ab52460-265341-anti-pan-cytokeratin-antibody-c-11-phycoerythrin-flow-cytometry.jpg "Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)")
Key features and details
- PE Mouse monoclonal [C-11] to pan Cytokeratin
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
PE Anti-pan Cytokeratin antibody [C-11]
pan Cytokeratin 一次抗体 製品一覧 -
製品の詳細
PE Mouse monoclonal [C-11] to pan Cytokeratin -
由来種
Mouse -
標識
PE. Ex: 488nm, Em: 575nm -
特異性
ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human pan Cytokeratin. Keratin-enriched preparation from human epidermoid carcinoma cell line A431
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ポジティブ･コントロール
- Flow Cyt (Intra): HT-29 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
C-11 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52460の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 1 µg/ml.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 1 µg/ml. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
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別名
- pan ck antibody
- pan-ck antibody
- panck antibody
画像
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Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)
Intracellular Flow Cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460.
Recommended conc. 0.5 µg/ml
Overlay with Isotype mouse IgG1 control.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab52460 は 18 報の論文で使用されています。
- Vaquero-Siguero N et al. Modeling Colorectal Cancer Progression Reveals Niche-Dependent Clonal Selection. Cancers (Basel) 14:N/A (2022). PubMed: 36077793
- Korangath P & Ivkov R Magnet-assisted Flow Cytometry of in vivo Tumors to Quantitate Cell-specific Responses to Magnetic Iron Oxide Nanoparticles. Bio Protoc 10:e3822 (2020). PubMed: 33659474
- Cheng KS et al. ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells. Theranostics 10:11026-11048 (2020). PubMed: 33042268
- Karin O et al. Senescent cell turnover slows with age providing an explanation for the Gompertz law. Nat Commun 10:5495 (2019). PubMed: 31792199
- Singh M et al. Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration. Cancer Res 79:2962-2977 (2019). PubMed: 30952634