PE Anti-MMP14 抗体 [EP1264Y] (ab303602)
![Flow Cytometry - PE Anti-MMP14 antibody [EP1264Y] (ab303602)](https://www.abcam.co.jp/ps/products/303/ab303602/Images/ab303602-2-anti-mmp14-antibody-ep1264y-pe-a431-flow-cytometry.jpg "Flow Cytometry - PE Anti-MMP14 antibody [EP1264Y] (ab303602)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP1264Y] to MMP14
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
製品の概要
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製品名
PE Anti-MMP14 antibody [EP1264Y] -
製品の詳細
PE Rabbit monoclonal [EP1264Y] to MMP14 -
由来種
Rabbit -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow Cyt: A431 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1264Y -
アイソタイプ
IgG
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab303602の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
|
Flow Cyt
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. -
組織特異性
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase. -
細胞内局在
Membrane. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 4323 Human
- Omim: 600754 Human
- SwissProt: P50281 Human
- Unigene: 2399 Human
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別名
- Matrix metalloproteinase-14 antibody
- Membrane type 1 metalloprotease antibody
- Membrane-type matrix metalloproteinase 1 antibody
see all
画像
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Flow Cytometry - PE Anti-MMP14 antibody [EP1264Y] (ab303602)
Flow cytometry overlay histogram showing wild-type A431 (green line) and MMP14 knockout A431 cells (ab261890) stained with AB303602 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (AB303602) (1x106 in 100μl at 1μg/ml) for 30 min at 4°C.
Isotype control antibody Rabbit IgG (monoclonal) Phycoerythrin (ab209478) was used at the same concentration and conditions as the primary antibody (wild-type A431 - black line MMP14 knockout A431 - grey line). Unlabeled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 585/42 bandpass filter.
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![PE Anti-MMP14 antibody [EP1264Y] (ab303602)](https://www.abcam.co.jp/ps/products/303/ab303602/Images/ab303602-494259-MTOConjugatedRecombinantAntibodyBenefits.jpg)
PE Anti-MMP14 antibody [EP1264Y] (ab303602) -
![PE Anti-MMP14 antibody [EP1264Y] (AB303602)](https://www.abcam.co.jp/ps/products/303/ab303602/Images/AB303602-1-Benefits-of-Recombinant-antibodies.jpg)
PE Anti-MMP14 antibody [EP1264Y] (AB303602)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab303602 は論文での使用が確認できていません。