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PE Anti-MHC Class 1 H2 Db/H2-D1 抗体 [27-11-13] (ab25547)

Submit a review Q&A (1)References (1)
Flow Cytometry - PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (ab25547)

    Key features and details

    • PE Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    FITC Unconjugated

    製品の概要

    • 製品名

      PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]
      MHC Class 1 H2 Db/H2-D1 一次抗体 製品一覧

    • 製品の詳細

      PE Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1

    • 由来種

      Mouse

    • 標識

      PE. Ex: 488nm, Em: 575nm

    • 特異性

      ab25547 recognizes the alpha3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Mouse

    • 免疫原

      Tissue, cells or virus corresponding to Mouse MHC Class 1 H2 Db/H2-D1. BDF1 mouse splenocytes

    • エピトープ

      This antibody reacts with an epitope on the alpha chain [alpha3 domain] of H2Db.

    • ポジティブ･コントロール

      • Flow cyt: Mouse BALB/c splenocytes.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C. Store In the Dark.

    • バッファー

      pH: 7.3
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 16% Sucrose

      Also contains a stabilizing agent.
    • Concentration information loading...

    • 精製度

      Affinity purified

    • ポリ/モノ

      モノクローナル

    • クローン名

      27-11-13

    • アイソタイプ

      IgG2a

    • 軽鎖の種類

      kappa

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab25547の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use 0.2µg for 106 cells.
    特記事項
    Flow Cyt
    Use 0.2µg for 106 cells.

    ターゲット情報

    • 関連性

      In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.

    • 細胞内局在

      Cell Membrane; single pass type I membrane protein

    • 参照データベース

      • 別名

        • H 2D cell surface glycoprotein antibody
        • H2 class I histocompatibility antigen, DB alpha chain antibody
        • H2D(B) antibody
        • H2D1 antibody
        • Histocompatibility 2, D region locus 1 antibody
        see all

      画像

      • Flow Cytometry - PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (ab25547)
        Flow Cytometry - PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (ab25547)

        Flow cytometric analysis of mouse BALB/c mouse splenocytes stained for MHC Class 1 H2 Db/H2-D1 using ab25547 at 0.2 μg/106 cells.

      参考文献 (1)

      ab25547 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab25547 は 1 報の論文で使用されています。

      • Hoerter JA  et al. Coreceptor affinity for MHC defines peptide specificity requirements for TCR interaction with coagonist peptide-MHC. J Exp Med 210:1807-21 (2013). PubMed: 23940257

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Question

      I want to use ab25547 and ab25588 which are primary antibodies to mouse MHCI proteins on fresh 10uM cryostat sections from mouse brain on slides that will be acetone fixed. Do you have a protocol for the acetone fixation step? I believe these antibodies are already conjucated to phycoerythrin. I have not used this before. Do I just do a protocol for primary antibody reaction, mount with gel mount (ab8214)then look under the scope with the FITC cube and look for yellow-orange cells? If so, do you have a protocol for this? Or should I use your ab8649 ABC kit? If so,then how? I guess I'm confused because the primary comes already conjugated to PE (probably for Facs).

      Read More
      Answer

      Thank you for your enquiry. We do not have a protocol specifically for acetone fixation. However, may I recommend the excellent resource at IHC world. The following link details technical tips to consider when performing acetone fixation. http://www.ihcworld.com/_technical_tips/crystat_section_ihc.htm Both ab25547 and ab25588 are directly conjugated with phycoerythrin and therefore will not require a secondary antibody to detect them. You will not require ab8649. Yes, you are correct you will only need to perform the primary antibody incubation i.e. Omit steps 9-14 from the ABC Immunohistochemical Staining protocol located at the following link. https://www.abcam.com/index.html?pageconfig=view_protocol&pid=158 Phycoerythrin or PE is excited by a similar wavelength to FITC (488nm) but emits at 578nm as opposed to 519nm (FITC). Therefore Im not certain you would see much emission using a FITC filter. It may be necessary to modify the detection wavelength if possible to detect a wavelength of 578nm. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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