PE Anti-LILRB1 抗体 [GHI/75], prediluted (ab186074)
![Flow Cytometry - PE Anti-LILRB1 antibody [GHI/75], prediluted (ab186074)](https://www.abcam.co.jp/ps/products/186/ab186074/Images/ab186074-1-anti-cd85j-LILRB1-antibody-ghi75-flow-cytometry-peripheral-blood-human.jpg "Flow Cytometry - PE Anti-LILRB1 antibody [GHI/75], prediluted (ab186074)")
Key features and details
- PE Mouse monoclonal [GHI/75] to LILRB1, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
PE Anti-LILRB1 antibody [GHI/75], prediluted
LILRB1 一次抗体 製品一覧 -
製品の詳細
PE Mouse monoclonal [GHI/75] to LILRB1, prediluted -
由来種
Mouse -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human LILRB1. Hairy cell leukaemia cells.
Database link: Q8NHL6 -
ポジティブ･コントロール
- Flow Cyt: Human blood cells.
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特記事項
ab186074 recognizes LILRB1, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading... -
精製度
Size exclusion -
ポリ/モノ
モノクローナル -
クローン名
GHI/75 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186074の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
ターゲット情報
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機能
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. -
組織特異性
Expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. Detected on a low percentage of T-cells and natural killer (NK) cells. -
配列類似性
Contains 4 Ig-like C2-type (immunoglobulin-like) domains. -
ドメイン
Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
翻訳後修飾
Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10859 Human
- Omim: 604811 Human
- SwissProt: Q8NHL6 Human
- Unigene: 667388 Human
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別名
- CD85 antibody
- CD85 antigen antibody
- CD85 antigen like family member J antibody
see all
画像
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Flow Cytometry - PE Anti-LILRB1 antibody [GHI/75], prediluted (ab186074)Flow cytometry analysis of human peripheral blood labeling CD85j/LILRB1 using ab186074 at 10 µL/100 µL whole blood. Surface staining.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab186074 は 1 報の論文で使用されています。
- Albayati Z et al. The Influence of Cytomegalovirus on Expression of HLA-G and its Ligand KIR2DL4 by Human Peripheral Blood Leucocyte Subsets. Scand J Immunol 86:396-407 (2017). PubMed: 28817184
