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PE Anti-HLA Class I 抗体 [MEM-123] (ab58998)

Submit a review Q&A (1)References (1)
Flow Cytometry - PE Anti-HLA Class I antibody [MEM-123] (ab58998)

    Key features and details

    • PE Mouse monoclonal [MEM-123] to HLA Class I
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG3

    Conjugates logo Related conjugates and formulations

    Unconjugated

    製品の概要

    • 製品名

      PE Anti-HLA Class I antibody [MEM-123]
      HLA Class I 一次抗体 製品一覧

    • 製品の詳細

      PE Mouse monoclonal [MEM-123] to HLA Class I

    • 由来種

      Mouse

    • 標識

      PE. Ex: 488nm, Em: 575nm

    • 特異性

      ab58998 reacts with all human classical MHC Class I molecules in native cell-surface forms as well as with human HLA G cDNA transfected cells. ab58998 completely blocks binding of classical W6/32 to surface expressed HLA G.

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Tissue, cells or virus corresponding to Human HLA Class I. COS-7 cells
      Database link: P04439

    • ポジティブ･コントロール

      • Flow Cyt: K562 cells

    • 特記事項

      Do not use after expiration date stamped on vial label. Short term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

      The purified antibody was conjugated with R Phycoerythrin (PE) under optimum conditions. The conjugate was purified by size exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      pH: 7.4
      Preservative: 0.097% Sodium azide
      Constituent: 0.2% BSA

      0.2% high grade protease free BSA
    • Concentration information loading...

    • 精製度

      Size exclusion

    • 特記事項（精製）

      The antibody was >95% pure as determined from SDS-PAGE (purified using protein A affinity chromatography prior to conjugation).

    • ポリ/モノ

      モノクローナル

    • クローン名

      MEM-123

    • アイソタイプ

      IgG3

    • 研究分野

    関連製品

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab58998の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use a concentration of 2 - 4 µg/ml.
    特記事項
    Flow Cyt
    Use a concentration of 2 - 4 µg/ml.

    ターゲット情報

    画像

    • Flow Cytometry - PE Anti-HLA Class I antibody [MEM-123] (ab58998)
      Flow Cytometry - PE Anti-HLA Class I antibody [MEM-123] (ab58998)
      Flow Cytometry analysis of K562 cells labeling HLA Class I with Anti-HLA Class I antibody [MEM-123] (Phycoerythrin) (ab58998).

    参考文献 (1)

    ab58998 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab58998 は 1 報の論文で使用されています。

    • Toyoshima K  et al. Analysis of circulating tumor cells derived from advanced gastric cancer. Int J Cancer 137:991-8 (2015). Flow Cyt ; Human . PubMed: 25622566

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Would like to use this antibody in IHC-Fr to stain human cells injected into mouse heart tissue.

    Read More
    Answer


    DISCOUNT CODE: ***
    Expiration date: June 22, 2012

    This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for ab58998 in IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com