AB83214

PE Anti-Heme Oxygenase 1 抗体 [HO-1-2]

Name: PE Anti-Heme Oxygenase 1 抗体 [HO-1-2] (ab83214) | Abcam
Brand: Abcam Limited
SKU: AB83214
Price: 73000 JPY
Availability: InStock

PE Anti-Heme Oxygenase 1 antibody [HO-1-2]

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(6 Publications)

Mouse Monoclonal Heme Oxygenase 1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Hmox1.

別名を表示する

HO, HO1, HMOX1, Heme oxygenase 1, HO-1

1 Images

Flow Cytometry (Intracellular) - PE Anti-Heme Oxygenase 1 antibody [HO-1-2] (AB83214)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-Heme Oxygenase 1 antibody [HO-1-2] (AB83214)

10⁶ Jurkat cells stained using ab83214 at a concentration of 10 μg/mL.

This image was generated using the ascites version of the product.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

HO-1-2

アイソタイプ

IgG2b

標識

励起波長/蛍光波長

Ex: 480;565nm, Em: 578nm

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt (Intra)

applications

免疫原

Native Full Length Protein corresponding to Rat Hmox1.

P06762

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/pe-mouse-igg2b-plpv219-isotype-control-ab91532'>ab91532</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

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製品の詳細

This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

Preservative: 0.09% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.

Biological function summary

Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.

Pathways

Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.

Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed : 11121422, PubMed : 19556236, PubMed : 7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed : 20055707).. Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

See full target information HMOX1

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 14:3983-4000 PubMed39309491

2024

Heme oxygenase 1-mediated ferroptosis in Kupffer cells initiates liver injury during heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Ru Li,Riqing Wei,Chenxin Liu,Keying Zhang,Sixiao He,Zhifeng Liu,Junhao Huang,Youyong Tang,Qiyuan An,Ligen Lin,Lishe Gan,Liying Zhao,Xiaoming Zou,Fudi Wang,Yuan Ping,Qiang Ma

Cancer immunology, immunotherapy : CII 72:2233-2244 PubMed36869896

2023

M2 tumor-associated macrophages resist to oxidative stress through heme oxygenase-1 in the colorectal cancer tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Misato Ito,Kosaku Mimura,Shotaro Nakajima,Hirokazu Okayama,Katsuharu Saito,Takahiro Nakajima,Tomohiro Kikuchi,Hisashi Onozawa,Shotaro Fujita,Wataru Sakamoto,Motonobu Saito,Tomoyuki Momma,Zenichiro Saze,Koji Kono

Journal of immunology (Baltimore, Md. : 1950) 206:1493-1504 PubMed33627377

2021

Conserved Epigenetic Programming and Enhanced Heme Metabolism Drive Memory B Cell Reactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Madeline J Price,Christopher D Scharer,Anna K Kania,Troy D Randall,Jeremy M Boss

Cell death and differentiation 24:1224-1238 PubMed28524859

2017

Mesenchymal stem cells sense mitochondria released from damaged cells as danger signals to activate their rescue properties.

Applications

Flow Cyt

Species

Human

Meriem Mahrouf-Yorgov,Lionel Augeul,Claire Crola Da Silva,Maud Jourdan,Muriel Rigolet,Sylvie Manin,René Ferrera,Michel Ovize,Adeline Henry,Aurélie Guguin,Jean-Paul Meningaud,Jean-Luc Dubois-Randé,Roberto Motterlini,Roberta Foresti,Anne-Marie Rodriguez

Scientific reports 7:755 PubMed28389660

2017

Characteristics of the heme catabolic pathway in mild unconjugated hyperbilirubinemia and their associations with inflammation and disease prevention.

Applications

Flow Cyt

Species

Human

Christine Mölzer,Marlies Wallner,Carina Kern,Anela Tosevska,René Zadnikar,Daniel Doberer,Rodrig Marculescu,Karl-Heinz Wagner

Endocrinology 153:4367-79 PubMed22733969

2012

Immunoregulatory natural killer cells suppress autoimmunity by down-regulating antigen-specific CD8+ T cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Margret Ehlers,Claudia Papewalis,Wiebke Stenzel,Benedikt Jacobs,Klaus L Meyer,René Deenen,Holger S Willenberg,Sven Schinner,Annette Thiel,Werner A Scherbaum,Evelyn Ullrich,Laurence Zitvogel,Matthias Schott

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Heme Oxygenase 1 抗体 [HO-1-2]

PE Anti-Heme Oxygenase 1 antibody [HO-1-2]

Flow Cytometry (Intracellular) - PE Anti-Heme Oxygenase 1 antibody [HO-1-2] (AB83214)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

代替製品

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組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise