PE Anti-HA tag 抗体 [EPR22819-101] (ab303586)
![Flow Cytometry (Intracellular) - PE Anti-HA tag antibody [EPR22819-101] (ab303586)](https://www.abcam.co.jp/ps/products/303/ab303586/Images/ab303586-2-anti-ha-tag-antibody-epr22819-101-pe-hek293t-transfected-with-tata-box-flow-cytometry.jpg "Flow Cytometry (Intracellular) - PE Anti-HA tag antibody [EPR22819-101] (ab303586)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR22819-101] to HA tag
- Suitable for: Flow Cyt (Intra)
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
製品の概要
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製品名
PE Anti-HA tag antibody [EPR22819-101]
HA tag 一次抗体 製品一覧 -
製品の詳細
PE Rabbit monoclonal [EPR22819-101] to HA tag -
由来種
Rabbit -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow Cyt (Intra): HEK293T transfected with TATA-box-binding protein (WT) expression vector containing HA-tag positive cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22819-101 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab303586の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/500. |
ターゲット情報
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関連性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
別名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
画像
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Flow Cytometry (Intracellular) - PE Anti-HA tag antibody [EPR22819-101] (ab303586)
Flow cytometry staining of HEK-293T (human embryonic kidney) transfected with TATA-box-binding protein (WT) expression vector containing HA-tag positive cells (right) and negative wild-type HEK-293T cells (left) stained with AB303586. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (AB303586) (1x 106 in 100μl at 1μg/ml (1/500)) for 30 min at 22°C.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 585/42 bandpass filter.
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![PE Anti-HA tag antibody [EPR22819-101] (ab303586)](https://www.abcam.co.jp/ps/products/303/ab303586/Images/ab303586-494243-MTOConjugatedRecombinantAntibodyBenefits.jpg)
PE Anti-HA tag antibody [EPR22819-101] (ab303586) -
![PE Anti-HA tag antibody [EPR22819-101] (AB303586)](https://www.abcam.co.jp/ps/products/303/ab303586/Images/AB303586-1-Benefits-of-Recombinant-antibodies.jpg)
PE Anti-HA tag antibody [EPR22819-101] (AB303586)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab303586 は論文での使用が確認できていません。