PE Anti-FITC 抗体 (ab25539)
Key features and details
- PE Sheep polyclonal to FITC
- Suitable for: WB, ELISA, ICC/IF, Dot blot
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG
製品の概要
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製品名
PE Anti-FITC antibody
FITC 一次抗体 製品一覧 -
製品の詳細
PE Sheep polyclonal to FITC -
由来種
Sheep -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: WB, ELISA, ICC/IF, Dot blotmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule: Fluorescein Isothiocyanate (Isomer I).
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特記事項
Ab25539 shows cross absorption with mouse, human and rat serum proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent. -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Ab25539 is purified by affinity chromatography. -
一次抗体 備考
Ab25539 shows cross absorption with mouse, human and rat serum proteins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25539の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent dilution.
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ELISA |
Use at an assay dependent dilution.
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ICC/IF |
Use µg for 10 cells.
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Dot blot |
Use at an assay dependent dilution.
|
特記事項 |
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WB
Use at an assay dependent dilution. |
ELISA
Use at an assay dependent dilution. |
ICC/IF
Use µg for 10 cells. |
Dot blot
Use at an assay dependent dilution. |
ターゲット情報
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関連性
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. -
別名
- Fluorescein Isothiocyanate antibody
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab25539 は 2 報の論文で使用されています。
- Zhang D et al. Crocin alleviates lipopolysaccharide-induced acute respiratory distress syndrome by protecting against glycocalyx damage and suppressing inflammatory signaling pathways. Inflamm Res 69:267-278 (2020). PubMed: 31925528
- Ma X et al. Fraxin Alleviates LPS-Induced ARDS by Downregulating Inflammatory Responses and Oxidative Damages and Reducing Pulmonary Vascular Permeability. Inflammation 42:1901-1912 (2019). PubMed: 31273573