JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB157300

PE/Cy5® Anti-CD3 抗体 [UCHT1], prediluted

PE/Cy5® Anti-CD3 antibody [UCHT1]

Be the first to review this product! Submit a review

|

(7 Publications)

Anti-CD3 antibody [UCHT1] conjugated to PE/Cy5® - suitable for flow cytometry, intracellular flow cytometry, and reacts with human and rat samples. Clone [UCHT1] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

Design your panel with our interactive flow cytometry panel builder. Click here.

別名を表示する

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

1 Images
Flow Cytometry - PE/Cy5® Anti-CD3 antibody [UCHT1] (AB157300)
  • Flow Cyt

Unknown

Flow Cytometry - PE/Cy5® Anti-CD3 antibody [UCHT1] (AB157300)

Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) PE-CyTM5.

関連する標識済み抗体及び組成の異なる製品 (13)

  • 660 APC

    APC Anti-CD3 antibody [UCHT1]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD3 antibody [UCHT1]

  • Biotin

    Biotin Anti-CD3 antibody [UCHT1]

  • 519 FITC

    FITC Anti-CD3 epsilon antibody [UCHT1]

  • 519 FITC

    FITC Anti-CD3 antibody [UCHT1]

  • 578 PE

    PE Anti-CD3 epsilon antibody [UCHT1]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD3 antibody [UCHT1]

  • 675 PerCP

    PerCP Anti-CD3 antibody [UCHT1]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD3 antibody [UCHT1]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD3e antibody [UCHT1]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD3e antibody [UCHT1]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD3 antibody [UCHT1]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD3 antibody [UCHT1]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

UCHT1

アイソタイプ

IgG1

標識

PE/Cy5®

励起波長/蛍光波長

Ex: 496nm, Em: 667nm

キャリアフリー

No

交差種

Rat, Human

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

P04234

エピトープ

ab157300 reacts with the epsilon chain of the CD3 complex.

style
left-column

代替製品

Primary Antibodies

AB135372

Anti-CD3 antibody [SP162]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)

  • 4
  • 10
Primary Antibodies

AB16044

Anti-CD3 antibody

Immunoprecipitation - Anti-CD3 antibody (AB16044)

  • 4
  • 2
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
Primary Antibodies

AB21703

Anti-CD3 epsilon antibody [SP7], prediluted

BOND RX™ Validated|RabMAb|Lab Essentials|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7], prediluted (AB21703)

  • 5
  • 1
Primary Antibodies

AB245731

Anti-CD3 antibody [SP162] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - BSA and Azide free (AB245731)

  • 5
  • 3
Primary Antibodies

AB246799

Anti-CD3 antibody [SP162] - Low endotoxin, Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (AB246799)

  • 0
  • 0
Primary Antibodies

AB5690

Anti-CD3 epsilon antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (AB5690)

  • 4
  • 46

組合せ製品

Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

  • 5
  • 1
Primary Antibodies

AB307134

Alexa Fluor® 488 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD3 antibody [SP162] (AB307134)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p><p><a href='/products/primary-antibodies/pe-cy5-mouse-igg1-kappa-monoclonal-15h6-isotype-control-ab67435'>ab67435</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p><p><a href='/products/primary-antibodies/pe-cy5-mouse-igg1-kappa-monoclonal-15h6-isotype-control-ab67435'>ab67435</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Size-exclusion chromatography
精製に関する特記事項
Purified antibody is conjugated with tandem dye PE/Cy5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
バッファー組成
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
style
left-column
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
style
left-column

文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Virulence 16:2457967 PubMed39981650

2025

Human SLAM-adapted canine distemper virus can enter human peripheral blood mononuclear cells and replicate in mice expressing human SLAM and defective for STAT1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Boyu Zhai,Wei Ran,Yiyang Sun,Angelita Alcos,Mengjia Liu,Jie Chen,Christopher D Richardson,Dongbo Sun,Jianjun Zhao

International archives of allergy and immunology 184:85-97 PubMed36265457

2022

Changes of T Lymphocyte Subsets in Peripheral Blood of Patients with Intermediate and Advanced Cervical Cancer before and after Nimotuzumab Combined with Chemoradiotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Teng,Guimin Cui,Ligang Qian,Lujun Zhao

Oncology letters 12:4087-4093 PubMed27895776

2016

Establishment of specific cytotoxic T lymphocyte culture system and its inhibitory effect on ovarian cancer.

Applications

Flow Cyt

Species

Human

Mingxing Sui,Lihui Si,Tianmin Xu,Manhua Cui

The Journal of experimental medicine 204:2537-44 PubMed17923503

2007

Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies.

Applications

Unspecified application

Species

Unspecified reactive species

Gabrielle M Siegers,Mahima Swamy,Edgar Fernández-Malavé,Susana Minguet,Sylvia Rathmann,Alberto C Guardo,Verónica Pérez-Flores,Jose R Regueiro,Balbino Alarcón,Paul Fisch,Wolfgang W A Schamel

The New England journal of medicine 354:1913-21 PubMed16672702

2006

Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Frédéric Rieux-Laucat,Claire Hivroz,Annick Lim,Véronique Mateo,Isabelle Pellier,Françoise Selz,Alain Fischer,Françoise Le Deist

Proceedings of the National Academy of Sciences of 101:16268-73 PubMed15534202

2004

Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly L Arnett,Stephen C Harrison,Don C Wiley

Journal of immunology (Baltimore, Md. : 1950) 161:1113-22 PubMed9686569

1998

Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

S le Gouvello,V Manceau,A Sobel
View all publications
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com