PE Anti-CD86 抗体 [EPR22958-106] (ab275357)
![Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (PE) (ab275357)](https://www.abcam.co.jp/ps/products/275/ab275357/Images/ab275357-1-anti-cd86-rpe-mouse-splenocytes-cells-flow-cyt.jpg "Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (PE) (ab275357)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR22958-106] to CD86
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
製品の概要
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製品名
PE Anti-CD86 antibody [EPR22958-106]
CD86 一次抗体 製品一覧 -
製品の詳細
PE Rabbit monoclonal [EPR22958-106] to CD86 -
由来種
Rabbit -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow cyt: C57 BL/6 mouse splenocytes treated with 1 µg/ml LPS for 3 days.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22958-106 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab275357の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/500.
|
| 特記事項 |
|---|
|
Flow Cyt
1/500. |
ターゲット情報
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機能
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
組織特異性
Expressed by activated B-lymphocytes and monocytes. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12524 Mouse
- SwissProt: P42082 Mouse
- Unigene: 1452 Mouse
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別名
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
画像
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Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (PE) (ab275357)
Flow cytometry staining of C57 BL/6 mouse splenocytes (top) or C57 BL/6 mouse splenocytes treated with 1 μg/ml LPS for 3 days (bottom), with ab275357 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). C57 BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab275357) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (1x 106 in 100μl at 1 µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD19.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/16 bandpass filter. Events were gated on live lymphocytes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab275357 は 1 報の論文で使用されています。
- Wei Q et al. LncRNA MEG3 Inhibits Tumor Progression by Modulating Macrophage Phenotypic Polarization via miR-145-5p/DAB2 Axis in Hepatocellular Carcinoma. J Hepatocell Carcinoma 10:1019-1035 (2023). PubMed: 37435155