PE Anti-CD81 抗体 [EPR21916] (ab283757)
![Flow Cytometry - PE Anti-CD81 antibody [EPR21916] (ab283757)](https://www.abcam.co.jp/ps/products/283/ab283757/Images/ab283757-1-anti-cd81-rpe-human-jurkat-cells-flow-cyt.jpg "Flow Cytometry - PE Anti-CD81 antibody [EPR21916] (ab283757)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR21916] to CD81
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
製品の概要
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製品名
PE Anti-CD81 antibody [EPR21916]
CD81 一次抗体 製品一覧 -
製品の詳細
PE Rabbit monoclonal [EPR21916] to CD81 -
由来種
Rabbit -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow cyt: Jurkat cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR21916 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab283757の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/2500.
|
| 特記事項 |
|---|
|
Flow Cyt
1/2500. |
ターゲット情報
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機能
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV. -
組織特異性
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines. -
関連疾患
Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
配列類似性
Belongs to the tetraspanin (TM4SF) family. -
翻訳後修飾
Not glycosylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 975 Human
- Omim: 186845 Human
- SwissProt: P60033 Human
- Unigene: 54457 Human
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別名
- 26 kDa cell surface protein TAPA 1 antibody
- 26 kDa cell surface protein TAPA-1 antibody
- 26 kDa cell surface protein TAPA1 antibody
see all
画像
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Flow Cytometry - PE Anti-CD81 antibody [EPR21916] (ab283757)
Flow cytometry overlay histogram showing left Jurkat positive cells and right negative HepG2 cells stained with ab283757 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab283757) (1x 106 in 100μl at 0.2μg/ml (1/2500)) for 30 min on ice.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab283757 は論文での使用が確認できていません。