AB216701

PE Anti-CD68 抗体 [FA-11]

PE Anti-CD68 antibody [FA-11]

Recombinant
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(2 Publications)

Rat Recombinant Monoclonal CD68 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Mouse samples. Cited in 2 publications.

別名を表示する

CD68, Macrosialin, Cd68

1 Images

Flow Cytometry (Intracellular) - PE Anti-CD68 antibody [FA-11] (AB216701)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-CD68 antibody [FA-11] (AB216701)

Overlay histogram showing Raw264.7 cells stained with ab216701 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab216701, 1/1000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in Raw264.7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

FA-11

アイソタイプ

IgG2a

標識

励起波長/蛍光波長

Ex: 480;565nm, Em: 578nm

キャリアフリー

交差種

Mouse

アプリケーション

Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<p><strong>The cellular localisation of this product has been verified in ICC/IF.</strong></p>" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

精製に関する特記事項

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Do Not Freeze|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.

Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

See full target information Cd68

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of hepatocellular carcinoma 11:543-562 PubMed38496248

2024

LncRNA MEG3 Reduces the Ratio of M2/M1 Macrophages Through the HuR/CCL5 Axis in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Huamei Wei,Xianjian Wu,Lizheng Huang,Chen Long,Qi Lu,Zheng Huang,Yanyan Huang,Wenchuan Li,Jian Pu

World journal of stem cells 12:500-513 PubMed32742567

2020

Cytotoxicity of nonylphenol on spermatogonial stem cells phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Hao Lei,Wen Yan,Chun-Hua Luo,Yu-Ming Guo,Yang-Yang Zhang,Xing-Huan Wang,Xin-Jun Su

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD68 抗体 [FA-11]

PE Anti-CD68 antibody [FA-11]

Flow Cytometry (Intracellular) - PE Anti-CD68 antibody [FA-11] (AB216701)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

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