PE Anti-CD59 抗体 [MEM-43] (ab36421)
![Flow Cytometry - PE Anti-CD59 antibody [MEM-43] (ab36421)](https://www.abcam.co.jp/ps/products/36/ab36421/Images/ab36421-278880-anti-cd59-antibody-mem-43-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CD59 antibody [MEM-43] (ab36421)")
Key features and details
- PE Mouse monoclonal [MEM-43] to CD59
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
PE Anti-CD59 antibody [MEM-43]
CD59 一次抗体 製品一覧 -
製品の詳細
PE Mouse monoclonal [MEM-43] to CD59 -
由来種
Mouse -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD59. Thymocytes and T lymphocytes
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エピトープ
ab36421 reacts with the well defined epitope (W40, R-53) on the CD59 molecule. -
ポジティブ･コントロール
- This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: JEG3.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
特記事項（精製）
The conjugate is purified by size-exclusion chromatography. -
ポリ/モノ
モノクローナル -
クローン名
MEM-43 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36421の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 20µl for 106 cells.
(or 100µl of whole blood).
ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
|
| ICC/IF |
1/100.
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| 特記事項 |
|---|
|
Flow Cyt
Use 20µl for 106 cells. (or 100µl of whole blood).
ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
|
ICC/IF
1/100. |
ターゲット情報
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機能
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. -
関連疾患
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300]. -
配列類似性
Contains 1 UPAR/Ly6 domain. -
翻訳後修飾
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. -
細胞内局在
Cell membrane. Secreted. Soluble form found in a number of tissues. - Information by UniProt
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参照データベース
- Entrez Gene: 966 Human
- Omim: 107271 Human
- SwissProt: P13987 Human
- Unigene: 278573 Human
- Unigene: 709466 Human
- Unigene: 710641 Human
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別名
- 16.3A5 antibody
- 1F5 antibody
- 1F5 antigen antibody
see all
画像
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Flow Cytometry - PE Anti-CD59 antibody [MEM-43] (ab36421)Flow Cytometry analysis of HL-60 (positive) and SP2 (negative) cells labeling CD59 with Anti-CD59 antibody [MEM-43] (Phycoerythrin) (ab36421).
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![Immunocytochemistry/ Immunofluorescence - PE Anti-CD59 antibody [MEM-43] (ab36421)](https://www.abcam.co.jp/ps/products/36/ab36421/Images/ab36421-48838-anti-cd59-antibody-mem-43-phycoerythrin-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - PE Anti-CD59 antibody [MEM-43] (ab36421)ab36421 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab36421 at 1 in 100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab36421 は 1 報の論文で使用されています。
- Caldwell ME et al. Cellular features of senescence during the evolution of human and murine ductal pancreatic cancer. Oncogene 31:1599-608 (2012). PubMed: 21860420