PE Anti-CD59 抗体 [MEM-43]

• Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD59 antibody [MEM-43] (AB36421)

ab36421 (concentration in sample 1 ug/ml, red-filled histogram) binds specifically to CD59 expressed on surface of HL60 cells (upper panel), but not to surface of SP2 cells (lower panel).

Level of non-specific binding was assessed using Mouse IgG2a isotype control PE antibody (PPV-04) under same conditions (concentration in sample 1 µg/ml, black-dashed histogram).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - PE Anti-CD59 antibody [MEM-43] (AB36421)

ab36421 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab36421 at 1 in 100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• Flow Cyt

Unknown

Flow Cytometry - PE Anti-CD59 antibody [MEM-43] (AB36421)

Flow Cytometry analysis of HL-60 (positive) and SP2 (negative) cells labeling CD59 with Anti-CD59 antibody [MEM-43] (Phycoerythrin) (ab36421).