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PE Anti-CD52 抗体 [HI186] (ab36443)

Submit a review Q&A (1)
Flow Cytometry - PE Anti-CD52 antibody [HI186] (ab36443)

    Key features and details

    • PE Mouse monoclonal [HI186] to CD52
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2b

    製品の概要

    • 製品名

      PE Anti-CD52 antibody [HI186]
      CD52 一次抗体 製品一覧

    • 製品の詳細

      PE Mouse monoclonal [HI186] to CD52

    • 由来種

      Mouse

    • 標識

      PE. Ex: 488nm, Em: 575nm

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Tissue, cells or virus corresponding to Human CD52. Human tonsil

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      pH: 7.4
      Preservative: 0.097% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...

    • 特記事項（精製）

      The conjugate was purified by size-exclusion chromatography.

    • ポリ/モノ

      モノクローナル

    • クローン名

      HI186

    • アイソタイプ

      IgG2b

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab36443の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use 20µl for 106 cells.

    or 100 µl of whole blood.
    特記事項
    Flow Cyt
    Use 20µl for 106 cells.

    or 100 µl of whole blood.

    ターゲット情報

    • 機能

      May play a role in carrying and orienting carbohydrate, as well as having a more specific role.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Cambridge pathology 1 antigen antibody
        • CAMPATH 1 antibody
        • CAMPATH 1 antigen antibody
        • CAMPATH-1 antigen antibody
        • CD 52 antibody
        • CD52 antibody
        • CD52 antigen antibody
        • CD52 molecule antibody
        • CD52_HUMAN antibody
        • CDw52 antibody
        • CDW52 antigen antibody
        • Epididymal secretory protein E5 antibody
        • Epididymis secretory sperm binding protein Li 171mP antibody
        • HE 5 antibody
        • He5 antibody
        • Human epididymis-specific protein 5 antibody
        see all

      画像

      • Flow Cytometry - PE Anti-CD52 antibody [HI186] (ab36443)
        Flow Cytometry - PE Anti-CD52 antibody [HI186] (ab36443)

        Flow cytometry analysis of human peripheral blood cells labelling CD52 with ab36443.

      参考文献 (0)

      ab36443 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab36443 は論文での使用が確認できていません。

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Question

      I am writing regarding your CD52 antibody for FC. The CD52 molecule besides being present in leukocytes is also present in the surface of ejaculated human sperm. Therefore we wish to use antibodies against this molecule to perform cell sorting of sperm from semen samples, and then to remove the leukocytes with another suitable marker. Yet, some studies have shown that antibodies against different epitopes of the leukocyte CD52 molecule may not bind to the sperm CD52 variant. Specifically, antibodies against the CAMPATH-1G, 097 and 2E5 epitopes bind to sperm CD52, but antibodies against CF1D12 do not. Accordingly, I wish to enquire if your CD 52 Mouse Anti-Human antibody has been tested on human sperm, or what is its binding region in the CD52 molecule.

      Read More
      Answer

      Thank you for your enquiry. Looking at the reply we had from the originator of this product, it seems that they have not tested this antibody on human sperm. Meanwhile, for the binding region, this antibody HI186 does not bind to the pronase-treated antigen nor to synthetic peptides, so it is suggested that the antibody might recognizes the N-linked carbohydrate. I hope this information will be helpful. Should you require other assistance, please do not hesitate to contact us.

      Read More

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