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PE Anti-CD34 抗体 [4H11(APG)], prediluted (ab18228)

Reviews (1) Submit a question References (2)
Flow Cytometry - PE Anti-CD34 antibody [4H11(APG)], prediluted (ab18228)

    Key features and details

    • PE Mouse monoclonal [4H11(APG)] to CD34, prediluted
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    APC Unconjugated

    製品の概要

    • 製品名

      PE Anti-CD34 antibody [4H11(APG)], prediluted
      CD34 一次抗体 製品一覧

    • 製品の詳細

      PE Mouse monoclonal [4H11(APG)] to CD34, prediluted

    • 由来種

      Mouse

    • 標識

      PE. Ex: 488nm, Em: 575nm

    • 特異性

      The antibody reacts with Class III epitope on CD34 antibody. It completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Tissue, cells or virus corresponding to Human CD34. Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia

    • ポジティブ･コントロール

      • CML cell line MOLM-7.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab18228の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt (1)
    Use 20µl for 106 cells.

    A cell suspension volume of 100 µl is recommended.

     

     

     

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     
    特記事項
    Flow Cyt
    Use 20µl for 106 cells.

    A cell suspension volume of 100 µl is recommended.

     

     

     

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    ターゲット情報

    • 機能

      Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

    • 組織特異性

      Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

    • 配列類似性

      Belongs to the CD34 family.

    • 発生段階

      On early hematopoietic progenitor cells.

    • 翻訳後修飾

      Highly glycosylated.
      Phosphorylated on serine residues by PKC.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CD34 antibody
        • CD34 antigen antibody
        • CD34 molecule antibody
        • CD34_HUMAN antibody
        • Cluster designation 34 antibody
        • cluster of differentiation 34 antibody
        • Hematopoietic progenitor cell antigen CD34 antibody
        • HPCA1 antibody
        • Mucosialin antibody
        • OTTHUMP00000034733 antibody
        • OTTHUMP00000034734 antibody
        see all

      画像

      • Flow Cytometry - PE Anti-CD34 antibody [4H11(APG)], prediluted (ab18228)
        Flow Cytometry - PE Anti-CD34 antibody [4H11(APG)], prediluted (ab18228)

        ab18228 staining CD34 in KG-1a cell line by Flow Cytometery. Blue peak repersents negative control while red peak show signal with ab18228.

      参考文献 (2)

      ab18228 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab18228 は 2 報の論文で使用されています。

      • Sun KT  et al. Reciprocal regulation of ?-globin expression by exo-miRNAs: Relevance to ?-globin silencing in ß-thalassemia major. Sci Rep 7:202 (2017). PubMed: 28303002
      • Castro FV  et al. 5T4 oncofetal antigen is expressed in high risk of relapse childhood pre-B acute lymphoblastic leukemia and is associated with a more invasive and chemotactic phenotype. Leukemia 26:1487-98 (2012). Flow Cyt ; Human . PubMed: 22266911

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Flow Cytometry abreview for Anti-CD34 antibody [4H11(APG)] (Phycoerythrin), prediluted

       Good
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Fixation
      NONE
      Sample
      Human Cell (B cell)
      Specification
      B cell
      Permeabilization
      No
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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      Verified customer

      投稿 Feb 22 2011

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