AB326514

PE Anti-CD20 antibody [2H7]

Name: PE Anti-CD20 antibody [2H7] (ab326514) | Abcam
Brand: Abcam Limited
SKU: AB326514
Price: 35000 JPY
Availability: InStock

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Mouse Monoclonal CD20 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples.

別名を表示する

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

3 Images

Flow Cytometry - PE Anti-CD20 antibody [2H7] (AB326514)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD20 antibody [2H7] (AB326514)

Expression profiling on peripheral blood subsets using ab326514. Innate panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of ab326514 (2 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Innate) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10Ч diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD20 antibody [2H7] (AB326514)

ab326514 works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
ab326514 was used in concentration 2 µg/ml in stained blood sample (2 x 10^6 cells).

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD20 antibody [2H7] (AB326514)

Expression profiling on peripheral blood subsets using ab326514. Adaptive panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13 : 827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of ab326514 (2 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Adaptive) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10Ч diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

2H7

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

標識

励起波長/蛍光波長

Ex: 480;565nm, Em: 578nm

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt

applications

特異性

The mouse monoclonal antibody 2H7 recognizes an extracellular epitope on CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.</p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Size-exclusion chromatography

精製に関する特記事項

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

バッファー組成

pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA

出荷温度

Ambient - Can Ship with Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze|Store in the dark

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).

See full target information MS4A1

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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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PE Anti-CD20 antibody [2H7]