JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB77138

PE Anti-CD2 抗体 [LT2]

PE Anti-CD2 antibody [LT2]

Be the first to review this product! Submit a review

|

(1 Publication)

Anti-CD2 antibody [LT2] conjugated to PE - suitable for flow cytometry, and reacts with human samples. Clone [LT2] is a mouse monoclonal antibody cited in 8 publications.

Design your panel with our interactive flow cytometry panel builder. Click here.

別名を表示する

CD2, SRBC, T-cell surface antigen CD2, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5

3 Images
Flow Cytometry - PE Anti-CD2 antibody [LT2] (AB77138)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD2 antibody [LT2] (AB77138)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab77138 (20 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - PE Anti-CD2 antibody [LT2] (AB77138)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD2 antibody [LT2] (AB77138)

Separation of MOLT-4 cells stained using ab77138 (concentration in sample 4 μg/m, red-filled) from MOLT-4 cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 4 μg/ml, same as ab77138 concentration, black-dashed) in flow cytometry analysis (surface staining).

Flow Cytometry - PE Anti-CD2 antibody [LT2] (AB77138)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD2 antibody [LT2] (AB77138)

Separation of human CD2 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab77138 (20 μl reagent / 100 μl of peripheral whole blood).

関連する標識済み抗体及び組成の異なる製品 (2)

  • 660 APC

    APC Anti-CD2 antibody [LT2]

  • 519 FITC

    FITC Anti-CD2 antibody [LT2]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

LT2

アイソタイプ

IgG2b

標識

PE

励起波長/蛍光波長

Ex: 480;565nm, Em: 578nm

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing CD2 protein. The exact immunogen used to generate this antibody is proprietary information.

P06729

特異性

The antibody reacts with CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.

style
left-column

代替製品

Primary Antibodies

AB119993

Anti-CD2 antibody [EPR3693]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-CD2 antibody [EPR3693] (AB119993)

  • 0
  • 0
Primary Antibodies

AB131276

Anti-CD2 antibody [EPR6451]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD2 antibody [EPR6451] (AB131276)

  • 0
  • 0
Primary Antibodies

AB219411

Anti-CD2 antibody [EPR21825]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] (AB219411)

  • 0
  • 0
Primary Antibodies

AB227698

Anti-CD2 antibody [SP304]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (AB227698)

  • 0
  • 0
Primary Antibodies

AB231059

Anti-CD2 antibody [EPR21825] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] - BSA and Azide free (AB231059)

  • 0
  • 0
Primary Antibodies

AB95556

PE/Cy5® Anti-CD2 antibody [RPA-2.10]

Flow Cytometry - PE/Cy5® Anti-CD2 antibody [RPA-2.10] (AB95556)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

  • 5
  • 1
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB208514

Alexa Fluor® 555 Anti-CD3D antibody [EP4426]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (AB208514)

  • 4
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 20 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.</p>" } } }
style
left-column

製品の詳細

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Size-exclusion chromatography
精製に関する特記事項
The conjugate is purified by size exclusion chromatography and adjusted for direct use. Purity >95% by SDS-PAGE.
バッファー組成
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD2 also known as T11 or LFA-2 acts as a cell surface glycoprotein expressed mainly on T-cells natural killer cells and thymocytes. This protein has a molecular mass of approximately 50-55 kDa. CD2 plays a critical role in facilitating cell-cell adhesion and signal transduction. It serves as a receptor for the CD58 ligand on antigen-presenting cells supporting the interaction between T-cells and these cells. By enhancing adhesion CD2 contributes to the formation of the immunological synapse necessary for effective immune response.
Biological function summary

The functioning of CD2 helps in the modulation of immune responses and T-cell activation. CD2 participates in signal transduction leading to cytokine production and proliferation. It is associated with the immunological synapse complex working closely with other surface molecules like Lck and CD3 to promote T-cell responses. This collaboration is vital for the development and function of immune cells driving adaptive immunity.

Pathways

CD2 engages in pathways integral to T-cell activation and signal transduction. A well-known pathway involving CD2 is the TCR (T-cell Receptor) signaling pathway where it partners with CD3 to initiate T-cell activation. Additionally CD2 influences the LAT (Linker for Activation of T-cells) pathway contributing to downstream signaling cascades that involve various signaling proteins such as Lck and ZAP-70 ensuring proper T-cell function.

CD2 shows connections to autoimmune diseases and cancers. For instance its involvement in abnormal T-cell activation links CD2 to autoimmune conditions like rheumatoid arthritis. Also various leukemias demonstrate altered CD2 expression suggesting a role in cancer pathogenesis. Through these disease states CD2's interaction with the CD58 protein becomes critical as this interaction affects the activity of T-cells in the disease milieu influencing disease progression and treatment outcomes.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
See full target information CD2
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Eksperimental'naia onkologiia 11:28-32 PubMed2737128

1989

[A panel of monoclonal antibodies against human lymphocyte antigens].

Applications

Unspecified application

Species

Unspecified reactive species

A V Filatov,P S Bachurin,N A Markova,N E Surnakova
View all publications
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com