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AB1168

PE Anti-CD19 抗体 [CB19]

PE Anti-CD19 antibody [CB19]

5

(1 Review)

|

(5 Publications)

Anti-CD19 antibody [CB19] conjugated to PE - suitable for flow cytometry, and reacts with human and Crab-Eating Macaque samples. Clone [CB19] is a mouse monoclonal antibody cited in 7 publications.

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別名を表示する

CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12

1 Images
Flow Cytometry - PE Anti-CD19 antibody [CB19] (AB1168)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD19 antibody [CB19] (AB1168)

Flow cytometry histogram showing 10µL of PE Anti-CD19 antibody [CB19] – (ab1168) used to stain 100µL of stabilized whole blood from healthy donor. A positive procedural control for T-lymphocyte, B-lymphocyte and NK cell enumeration (stabilized blood) was stained using mouse anti human CD19 RPE. Cells were gated on lymphocytes (using SSC/FSC dot plot). The first peak (near to the axis origin) shows negative cells (CD19-), the second peak shows the positive population, B cells according to the antibody specificity (CD19+).

関連する標識済み抗体及び組成の異なる製品 (2)

  • 660 APC

    APC Anti-CD19 antibody [CB19]

  • 519 FITC

    FITC Anti-CD19 antibody [CB19]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

CB19

アイソタイプ

IgG1

軽鎖のタイプ

unknown

標識

PE

励起波長/蛍光波長

Ex: 480;565nm, Em: 578nm

キャリアフリー

No

交差種

Human, Rhesus monkey, Macaque monkey, Chimpanzee

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing CD19 protein. The exact immunogen used to generate this antibody is proprietary information.

P15391

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. <a href='/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Chimpanzee": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. <a href='/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Macaque monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. <a href='/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Rhesus monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10ul of CD19 (PE) is sufficient for labelling of 1x10^6 cells. <a href='/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

製品の詳細

What is this antibody validated in?
PE Anti-CD19 antibody [CB19] (ab1168) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt) in Chimpanzee, Human, Macaque monkey, Rhesus monkey samples.

Related products
Antibody clone CB19 is also available pre-conjugated to a variety of labels for your convenience – Anti-CD19 PE [CB19] (ab1168).

Other related products
We have a range of other formats of antibody clone [CB19] also available for your convenience: ab254170, Carrier free - ab255925, Alexa Fluor® 647 - ab307266, FITC - ab1167, PE - ab1168

出荷温度及び保存条件

製品の状態
Liquid
バッファー組成
Preservative: 0.1% Sodium azide Constituents: 0.5% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.
Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed : 29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed : 1373518, PubMed : 16672701, PubMed : 2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126, PubMed : 9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed : 9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed : 1373518, PubMed : 2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126).
See full target information CD19

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:5037 PubMed39934157

2025

The antidiabetic effect of safflower yellow by regulating the GOAT/ghrelin/GHS-R1a/cAMP/TRPM2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxiao Ma,Haifeng Zhang,Qihui Yan,Ping Wang,Weiying Guo,Lu Yu

Circulation 138:1144-1154 PubMed29593014

2018

Ryanodine Receptor Calcium Leak in Circulating B-Lymphocytes as a Biomarker in Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Kushnir,Gaetano Santulli,Steven R Reiken,Ellie Coromilas,Sarah J Godfrey,Danielle L Brunjes,Paolo C Colombo,Melana Yuzefpolskaya,Seth I Sokol,Richard N Kitsis,Andrew R Marks

Nature communications 8:14930 PubMed28374737

2017

Extinct type of human parvovirus B19 persists in tonsillar B cells.

Applications

Flow Cyt

Species

Unspecified reactive species

Lari Pyöriä,Mari Toppinen,Elina Mäntylä,Lea Hedman,Leena-Maija Aaltonen,Maija Vihinen-Ranta,Taru Ilmarinen,Maria Söderlund-Venermo,Klaus Hedman,Maria F Perdomo

Journal of immunology (Baltimore, Md. : 1950) 149:2841-50 PubMed1383329

1992

The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules.

Applications

Unspecified application

Species

Unspecified reactive species

L E Bradbury,G S Kansas,S Levy,R L Evans,T F Tedder

Science (New York, N.Y.) 256:105-7 PubMed1373518

1992

CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

R H Carter,D T Fearon
View all publications

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