JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB234280

PE Anti-CD146 抗体 [P1H12]

PE Anti-CD146 antibody [P1H12]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal CD146 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing MCAM protein.

別名を表示する

CD146, MUC18, MCAM, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

1 Images
Flow Cytometry - PE Anti-CD146 antibody [P1H12] (AB234280)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD146 antibody [P1H12] (AB234280)

HUVEC cells. Gated on singlets.

関連する標識済み抗体及び組成の異なる製品 (3)

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

P1H12

アイソタイプ

IgG1

標識

PE

励起波長/蛍光波長

Ex: 480;565nm, Em: 578nm

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing MCAM protein. The exact immunogen used to generate this antibody is proprietary information.

P43121

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Dog": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Size-exclusion chromatography
精製に関する特記事項
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
バッファー組成
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze|Store in the dark
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD146 also known as MCAM or MUC18 is a cell adhesion molecule that belongs to the immunoglobulin superfamily. With a mass of approximately 113 kDa CD146 is expressed mainly on the surface of endothelial cells but it can also be found on smooth muscle cells some T-cells and in certain cancerous tissues. This protein plays a role in cell-cell adhesion contributing to the integrity and signaling functions within tissue microenvironments.
Biological function summary

CD146 influences the migration and organization of cells within the vascular system. It serves as a component of cell adhesion complexes facilitating interactions between endothelial cells and other cell types. Additionally CD146 contributes to angiogenesis the process by which new blood vessels form from existing vasculature which is critical during tissue development and repair. This function makes it an important element in maintaining normal physiological processes.

Pathways

CD146 participates in the Wnt/β-catenin and MAPK signaling pathways which are vital in cell proliferation and differentiation. Through the Wnt/β-catenin pathway CD146 interacts with other proteins like Frizzled receptors to regulate gene transcription. In the MAPK pathway it is linked with signaling cascades that involve proteins such as ERK leading to cellular responses necessary for growth and response to external stimuli.

CD146 has been implicated in melanoma progression and cardiovascular diseases. Its overexpression in melanoma associates it with increased tumor growth and metastasis often involving interactions with other adhesion molecules like integrins. In cardiovascular diseases CD146 relates to atherosclerosis as its expression on endothelial cells can promote inflammatory responses. Understanding these connections provides insights into potential therapeutic targets for treating these conditions.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
See full target information MCAM
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e36483 PubMed39253182

2024

Navitoclax safety, tolerability, and effect on biomarkers of senescence and neurodegeneration in aged nonhuman primates.

Applications

Unspecified application

Species

Unspecified reactive species

Edward F Greenberg,Martin J Voorbach,Alexandra Smith,David R Reuter,Yuchuan Zhuang,Ji-Quan Wang,Dustin W Wooten,Elizabeth Asque,Min Hu,Carolin Hoft,Ryan Duggan,Matthew Townsend,Karin Orsi,Karen Dalecki,Willi Amberg,Lori Duggan,Heather Knight,Joseph S Spina,Yupeng He,Kennan Marsh,Vivian Zhao,Suzanne Ybarra,Jennifer Mollon,Yuni Fang,Aparna Vasanthakumar,Susan Westmoreland,Mathias Droescher,Sjoerd J Finnema,Hana Florian
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB134065

Anti-CD146 antibody [EPR3207(2)]

RabMAb|Recombinant

Western blot - Anti-CD146 antibody [EPR3207(2)] (AB134065)

  • 4
  • 1
Primary Antibodies

AB196448

Alexa Fluor® 488 Anti-CD146 antibody [EPR3208]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD146 antibody [EPR3208] (AB196448)

  • 4
  • 1
Primary Antibodies

AB209298

PE Anti-CD146 antibody [EPR3208]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD146 antibody [EPR3208] (AB209298)

  • 0
  • 0
Primary Antibodies

AB210072

Anti-CD146 antibody [EPR3208] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (AB210072)

  • 4
  • 1
Primary Antibodies

AB216361

PerCP Anti-CD146 antibody [EPR3208]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PerCP Anti-CD146 antibody [EPR3208] (AB216361)

  • 0
  • 0
Primary Antibodies

AB217072

Biotin Anti-CD146 antibody [EPR3208]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-CD146 antibody [EPR3208] (AB217072)

  • 3
  • 1
Primary Antibodies

AB75769

Anti-CD146 antibody [EPR3208]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

  • 4
  • 41

組合せ製品

Assay Kits

AB315318

Human CD146 ELISA Kit

SimpleStep

Sandwich ELISA - Human CD146 ELISA Kit (AB315318)

  • 0
  • 0
Primary Antibodies

AB314277

PE Anti-CD105 antibody [EPR22811-18]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD105 antibody [EPR22811-18] (AB314277)

  • 0
  • 0
Primary Antibodies

AB200317

Alexa Fluor® 647 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD45 antibody [EP322Y] (AB200317)

  • 0
  • 1
Primary Antibodies

AB215912

Alexa Fluor® 647 Anti-CD31 antibody [JC/70A]

Immunocytochemistry - Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (AB215912)

  • 0
  • 1
Primary Antibodies

AB223930

PE Anti-CD34 antibody [EP373Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (AB223930)

  • 5
  • 1

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com