PE Anti-C5a-R 抗体 [EPR23278-117] (ab303604)
![Flow Cytometry - PE Anti-C5a-R antibody [EPR23278-117] (ab303604)](https://www.abcam.co.jp/ps/products/303/ab303604/Images/ab303604-2-anti-c5a-r-antibody-epr23278-117-pe-pbmc-flow-cytometry.jpg "Flow Cytometry - PE Anti-C5a-R antibody [EPR23278-117] (ab303604)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR23278-117] to C5a-R
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
製品の概要
-
製品名
PE Anti-C5a-R antibody [EPR23278-117]
C5a-R 一次抗体 製品一覧 -
製品の詳細
PE Rabbit monoclonal [EPR23278-117] to C5a-R -
由来種
Rabbit -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- Flow Cyt: Human PBMCs.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23278-117 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab303604の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/500.
|
| 特記事項 |
|---|
|
Flow Cyt
1/500. |
ターゲット情報
-
機能
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
翻訳後修飾
Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 728 Human
- Omim: 113995 Human
- SwissProt: P21730 Human
- Unigene: 2161 Human
-
別名
- ANAPHYLATOXIN RECEPTOR antibody
- C5a anaphylatoxin chemotactic receptor antibody
- C5a anaphylatoxin receptor antibody
see all
画像
-
Flow Cytometry - PE Anti-C5a-R antibody [EPR23278-117] (ab303604)
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with AB303604 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (AB303604) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (100 μl at 1μg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD11b.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 585/42 bandpass filter. Events were gated on live monocytes.
-
![PE Anti-C5a-R antibody [EPR23278-117] (ab303604)](https://www.abcam.co.jp/ps/products/303/ab303604/Images/ab303604-494261-MTOConjugatedRecombinantAntibodyBenefits.jpg)
PE Anti-C5a-R antibody [EPR23278-117] (ab303604) -
![PE Anti-C5a-R antibody [EPR23278-117] (AB303604)](https://www.abcam.co.jp/ps/products/303/ab303604/Images/AB303604-1-Benefits-of-Recombinant-antibodies.jpg)
PE Anti-C5a-R antibody [EPR23278-117] (AB303604)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab303604 は論文での使用が確認できていません。