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AB322528

PE Anti-Asialoglycoprotein Receptor 1/HL-1 抗体 [EPR22642-17]

PE Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]

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Rabbit Recombinant Monoclonal Asialoglycoprotein Receptor 1/HL-1 antibody - conjugated to PE. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples.

別名を表示する

CLEC4H1, ASGR1, Asialoglycoprotein receptor 1, ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1

1 Images
Flow Cytometry - PE Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (AB322528)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (AB322528)

Flow cytometric analysis of HEK-293 (human embryonic kidney epithelial cell, Left) / HepG2 (human hepatocellular carcinoma epithelial cell, Right) cells labelling Asialoglycoprotein Receptor 1/HL-1 with ab322528 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : HEK-293 (PMID : 34508778).
Gated on viable cells.

関連する標識済み抗体及び組成の異なる製品 (5)

  • Carrier free

    Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] - BSA and Azide free

  • Unconjugated

    Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]

  • HRP

    HRP Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22642-17

アイソタイプ

IgG

標識

PE

励起波長/蛍光波長

Ex: 480;565nm, Em: 578nm

キャリアフリー

No

交差種

Human, Human

アプリケーション

IHC-P, Flow Cyt, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/5000", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Asialoglycoprotein Receptor 1 (ASGR1) also known as ASGPR 1 or asialoglycoprotein receptor is a well-known protein with a critical role in the mechanism of recognizing and binding to asialoglycoproteins. It has a molecular mass of approximately 46 kDa. ASGR1 is expressed mostly in liver cells specifically hepatocytes. The unique characteristic of this receptor is its lectin-like nature which allows it to bind to specific sugar moieties present on desialylated glycoproteins. Through receptor-mediated endocytosis it facilitates their removal from the bloodstream.
Biological function summary

The function of ASGR1 extends significantly. It operates as part of the asialoglycoprotein receptor complex interfacing directly with the ligand proteins to mediate endocytosis. This process is essential in most mammalian systems for maintaining serum glycoprotein homeostasis. By clearing desialylated glycoproteins from circulation ASGR1 helps prevent potential adverse effects these molecules might cause like immune activation or interference with other biological functions.

Pathways

ASGR1 is integral to glycoprotein clearance pathways. It actively participates in the hepatic clearance pathway ensuring the regulation of serum glycoprotein levels. The removal of asialoglycoproteins involves interactions with several other hepatic proteins such as GalNAc transferase which can further facilitate the remodeling of glycoconjugates. Understanding these interactions is key for comprehending how the liver maintains overall metabolic balance.

ASGR1 is linked to certain liver-related conditions. Dysfunction or aberrant expression of the ASGR1 receptor can relate to elevated levels of serum glycoproteins which might be observed in liver cirrhosis. Elevated glycoproteins can exacerbate the progression of liver diseases. Furthermore ASGR1 protein interaction with viral glycoproteins has implications in hepatitis infections where it may inadvertently facilitate viral entry into hepatocytes affecting liver function and contributing to disease pathology.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transmembrane protein predominantly expressed on hepatocytes that plays a key role in endocytosis of plasma glycoproteins that lack terminal sialic acid residues. Specifically recognizes terminal galactose and N-acetylgalactosamine residues, facilitating the clearance of desialylated glycoproteins from circulation. Plays thereby a role in a variety of physiological processes, such as removal of desialylated platelets, elimination of activated lymphocytes and maintenance of serum glycoprotein homeostasis (PubMed : 25485912). Plays a role in the removal of desialylated platelets by activating a feedback loop regulating thrombopoietin (TPO) expression via the JAK2-STAT3 signaling pathway (PubMed : 25485912). Contributes also to recognition and elimination of activated lymphocytes by hepatocytes that can act as cytotoxic effectors (PubMed : 21656538). May also play a physiological role in the regulatory network of lipid homeostasis (By similarity). Upon ligand binding, the receptor-ligand complex is internalized and trafficked to a sorting organelle, where dissociation occurs. The receptor is then recycled back to the cell surface.. (Microbial infection) Plays an essential role in ACE2-independent SARS-CoV-2 entry by acting as alternate receptor.
See full target information ASGR1
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組合せ製品

Assay Kits

AB309431

Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit

Sandwich ELISA - Human Asialoglycoprotein Receptor 1/HL-1 ELISA kit (AB309431)

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