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リコンビナントRabMAb

Anti-PDZRN4 抗体 [EPR11852] (ab171083)

Submit a review Submit a question References (1)
Western blot - Anti-PDZRN4 antibody [EPR11852] (ab171083)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZRN4 antibody [EPR11852] (ab171083)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZRN4 antibody [EPR11852] (ab171083)
  • Flow Cytometry (Intracellular) - Anti-PDZRN4 antibody [EPR11852] (ab171083)
  • Anti-PDZRN4 antibody [EPR11852] (ab171083)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11852] to PDZRN4
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PDZRN4 antibody [EPR11852]
    PDZRN4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11852] to PDZRN4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
    適用なし: IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment within Human PDZRN4. The exact sequence is proprietary.
    Database link: Q6ZMN7

  • ポジティブ･コントロール

    • Human prostate, HepG2 and U87-MG lysates; Human cervical carcinoma and lung carcinoma tissues; U87-MG cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: BSA, Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR11852

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab171083の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 117 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 117 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 関連性

    PDZRN4 (PDZ domain-containing RING finger protein 4) contains two PDZ (DHR) domains and a RING-type zinc finger. Its function is unknown.

  • 参照データベース

    • 別名

      • 1110017D07Rik antibody
      • IMAGE5767589 antibody
      • KIAA3027 antibody
      • Ligand of Numb protein X 4 antibody
      • LNX 4 antibody
      • LNX4 antibody
      • mKIAA3027 antibody
      • PDZ domain containing ring finger 4 antibody
      • PDZ domain containing RING finger protein 4 antibody
      • PDZRN 4 antibody
      • SAMCAP3L antibody
      • SEMACAP3 like protein antibody
      • SEMCAP3L antibody
      see all

    画像

    • Western blot - Anti-PDZRN4 antibody [EPR11852] (ab171083)
      Western blot - Anti-PDZRN4 antibody [EPR11852] (ab171083)
      All lanes : Anti-PDZRN4 antibody [EPR11852] (ab171083) at 1/1000 dilution

      Lane 1 : Human prostate tissue lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 117 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZRN4 antibody [EPR11852] (ab171083)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZRN4 antibody [EPR11852] (ab171083)

      Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling PDZRN4 with ab171083 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZRN4 antibody [EPR11852] (ab171083)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZRN4 antibody [EPR11852] (ab171083)

      Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling PDZRN4 with ab171083 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-PDZRN4 antibody [EPR11852] (ab171083)
      Flow Cytometry (Intracellular) - Anti-PDZRN4 antibody [EPR11852] (ab171083)

      Intracellular flow cytometric analysis of permeabilized U87-MG cells labeling PDZRN4 with ab171083 at 1/100 dilution (red), compared to arabbit IgG negative control (green). 

       

       

    • Anti-PDZRN4 antibody [EPR11852] (ab171083)
      Anti-PDZRN4 antibody [EPR11852] (ab171083)

    参考文献 (1)

    ab171083 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab171083 は 1 報の論文で使用されています。

    • Jin P  et al. PDZRN4 suppresses tumorigenesis and androgen therapy-resistance in prostate cancer. J Cancer 13:2293-2300 (2022). PubMed: 35517421

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