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Anti-PDX1 抗体 [OTI2A12] (ab84987)

Submit a review Q&A (1)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987)
  • Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)
  • Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)
  • Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [OTI2A12] (ab84987)

Key features and details

  • Mouse monoclonal [OTI2A12] to PDX1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-PDX1 antibody [OTI2A12]
    PDX1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [OTI2A12] to PDX1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-283 of human PDX1 expressed in E. coli.

  • ポジティブ･コントロール

    • IHC-P: pancreas; carcinoma of pancreas WB: HEK293T cells transfected with PDX1; lysate from 293T cells transfected with PDX1 ICC/IF: HeLa cells transiently transfected by PDX1; HeLa cells

  • 特記事項

    The clone number has been updated from 2A12 to OTI2A12, both clone numbers name the same clone.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab84987の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/20000. Predicted molecular weight: 31 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
1/20000. Predicted molecular weight: 31 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.

  • 組織特異性

    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).

  • 関連疾患

    Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
    Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.

  • 配列類似性

    Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
    Contains 1 homeobox DNA-binding domain.

  • ドメイン

    The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
    The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).

  • 翻訳後修飾

    Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Glucose sensitive factor antibody
      • Glucose-sensitive factor antibody
      • GSF antibody
      • IDX 1 antibody
      • IDX-1 antibody
      • IDX1 antibody
      • Insulin promoter factor 1 antibody
      • Insulin promoter factor 1 homeodomain transcription factor antibody
      • Insulin upstream factor 1 antibody
      • IPF 1 antibody
      • IPF-1 antibody
      • IPF1 antibody
      • Islet/duodenum homeobox 1 antibody
      • Islet/duodenum homeobox-1 antibody
      • IUF 1 antibody
      • IUF-1 antibody
      • IUF1 antibody
      • MODY4 antibody
      • Pancreas/duodenum homeobox 1 antibody
      • Pancreas/duodenum homeobox protein 1 antibody
      • pancreatic and duodenal homeobox P antibody
      • PDX 1 antibody
      • PDX-1 antibody
      • PDX1 antibody
      • PDX1_HUMAN antibody
      • Somatostatin transactivating factor 1 antibody
      • Somatostatin-transactivating factor 1 antibody
      • STF 1 antibody
      • STF-1 antibody
      • STF1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987)
      ab84987, staining PDX1 in paraffin embedded human pancreas carcinoma tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [OTI2A12] (ab84987)
      ab84987, staining PDX1 in paraffin embedded human pancreas by Immunohistochemistry.
    • Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)
      Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)
      Anti-PDX1 antibody [OTI2A12] (ab84987) at 1/20000 dilution + lysate from HEK293T cells transfected with human PDX1 expression vector

      Predicted band size: 31 kDa



      HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200033)
    • Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)
      Western blot - Anti-PDX1 antibody [OTI2A12] (ab84987)
      All lanes : Anti-PDX1 antibody [OTI2A12] (ab84987)

      Lane 1 : lysate from HEK293T cells transfected with an empty expression vector
      Lane 2 : lysate from HEK293T cells transfected with a human PDX1 expression vector

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 31 kDa
      Observed band size: 31 kDa



      HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200033)
    • Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [OTI2A12] (ab84987)
      Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [OTI2A12] (ab84987)
      ab84987, staining PDX1 by Immunofluoresence in HeLa cells transiently transfected with a vector expressing PDX1.

    参考文献 (5)

    ab84987 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab84987 は 5 報の論文で使用されています。

    • Zhang X  et al. Micro-RNA-124-5p promotes insulin producing cell differentiation through regulating transcriptional factor NKX6.1. Biochem Biophys Rep 30:101273 (2022). PubMed: 35592615
    • Soltani A  et al. Treatment of diabetic mice by microfluidic system-assisted transplantation of stem cells-derived insulin-producing cells transduced with miRNA. Life Sci 274:119338 (2021). PubMed: 33716064
    • Jafarian A  et al. The Generation of Insulin Producing Cells from Human Mesenchymal Stem Cells by MiR-375 and Anti-MiR-9. PLoS One 10:e0128650 (2015). IF . PubMed: 26047014
    • Allahverdi A  et al. Differentiation of Human Mesenchymal Stem Cells into Insulin Producing Cells by Using A Lentiviral Vector Carrying PDX1. Cell J 17:231-42 (2015). WB . PubMed: 26199902
    • Jay CM  et al. Assessment of intravenous pbi-shRNA PDX1 nanoparticle (OFHIRNA-PDX1) in yucatan swine. Cancer Gene Ther 20:683-9 (2013). PubMed: 24287722

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Dear support team,
    I am interested in an antibody against chicken Pdx1. Since currently
    there is none, I would like to know wether your Anti-PDX1 antibody
    (ab47267) crossreacts with the chicken protein. Could you tell me the
    sequence of the immunogen so that I can check how is the conservation
    of the used epitope between mouse and chick?
    Thank you in andvance for your help!

    Read More
    Answer

    Thank you very much for contacting us.

    We currently do not have the immunogen sequence of ab47267 available, but I have contacted the originator for the information you requested, and I am awaiting a reply. I will forward to you the sequence as soon as I receive it.

    In the meantime I have checked our other PDX1 antibodies for their potential cross-reactivity with chicken. According to my BLAST research on NCBI or SwissProt(http://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE_TYPE=BlastHomeand http://www.uniprot.org/uniprot/), the immunogen sequence of ab94931 reveals a sequence similarity of 100% to the chicken orthologue, and thus should cross-react:

    https://www.abcam.com/PDX1-antibody-ab94931.html (or use the following: https://www.abcam.com/PDX1-antibody-ab94931.html).

    Please note that this antibody is a fast-track antibody: These antibodies have undergone variable degrees of characterization. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast-track antibodiesare released quickly to meet customer needs and requirements; the products are still undergoing further tests and characterization steps. Therefore, we release Fast-Track antibodies at a reduced price. Please read the terms of use on the datasheet for further information.

    Also, I have checked the following antibodies:

    ab84987: 72% to SwissProt E1C2J1. Candidate for testing? Please contact us, as you might be eligible for our testing discount program (https://www.abcam.com/collaborationdiscount).

    ab19385: 60% sequence similarity. Unlikely cross-reactivity.

    ab98298 (immunogen AFQRGPAPEFSASP): 57% to chicken PDX1, but 100% to a number of other proteins, so cross-reactivity to PDX1 is unlikely, staining of other unrelated proteins likely. Therefore, not suitable for testing.

    I hope this is of help to you. Please let me know if I can be of any further assistance.

    Read More

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