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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PDPK1 抗体 [EP569Y] (ab52893)

Submit a review Submit a question References (27)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody [EP569Y] (ab52893)
  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody [EP569Y] (ab52893)
  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
  • Anti-PDPK1 antibody [EP569Y] (ab52893)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP569Y] to PDPK1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, Pig

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PDPK1 antibody [EP569Y]
    PDPK1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP569Y] to PDPK1

  • 由来種

    Rabbit

  • 特異性

    This antibody detects PDK1 both when phosphorylated and non-phosphorylated on Tyrosine 373 and 376.

    The mouse, rat and pig recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and pig.

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human, Pig

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: PC-12, Rat testis, HEK-293, 22Rv1, PIEC, HeLa, K-562, and F9 lysates. IHC-P: human lung carcinoma and human prostate carcinoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab52893の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 63 kDa).
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse, rat and pig recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and pig.

See IHC antigen retrieval protocols.
特記事項
WB
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 63 kDa).
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse, rat and pig recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and pig.

See IHC antigen retrieval protocols.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.

  • 組織特異性

    Appears to be expressed ubiquitously.

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.

  • 細胞内局在

    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 3 phosphoinositide dependent protein kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK1 antibody
    • Pdpk1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PDPK2P antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody [EP569Y] (ab52893)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody [EP569Y] (ab52893)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling PDPK1 with Purified ab52893 at 1:150 dilution(2.4 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
    Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PDPK1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HEK293 cell lysate (20 µg)
    Lane 4: MCF7 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab52893 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab52893 was shown to recognize PDPK1 when PDPK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PDPK1 knockout samples were subjected to SDS-PAGE. ab52893 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
    Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
    Anti-PDPK1 antibody [EP569Y] (ab52893) at 1/1000 dilution (Purified) + F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

    Predicted band size: 63 kDa
    Observed band size: 52-65 kDa why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in PMID: 30171166

  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
    Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
    All lanes : Anti-PDPK1 antibody [EP569Y] (ab52893) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

    Predicted band size: 63 kDa
    Observed band size: 52-65 kDa why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in PMID: 30171166

  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
    Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
    All lanes : Anti-PDPK1 antibody [EP569Y] (ab52893) at 1/1000 dilution (Purified)

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 2 : Rat testis lysates
    Lane 3 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 4 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates
    Lane 5 : PIEC (PIG iliac artery endothelial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa
    Observed band size: 52-65 kDa why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in PMID: 30171166

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody [EP569Y] (ab52893)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody [EP569Y] (ab52893)

    Ab52893 (1:100) staining human PDPK1 in human prostate carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
    Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
    Anti-PDPK1 antibody [EP569Y] (ab52893) at 1/1000 dilution + 293 cell lysate

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

  • Anti-PDPK1 antibody [EP569Y] (ab52893)
    Anti-PDPK1 antibody [EP569Y] (ab52893)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (27)

ab52893 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab52893 は 27 報の論文で使用されています。

  • Meraz IM  et al. 3-Phosphoinositide-dependent kinase 1 drives acquired resistance to osimertinib. Commun Biol 6:509 (2023). PubMed: 37169941
  • Oh J  et al. Embryonic vitamin D deficiency programs hematopoietic stem cells to induce type 2 diabetes. Nat Commun 14:3278 (2023). PubMed: 37311757
  • Wu S  et al. Telomerase RNA TERC and the PI3K-AKT pathway form a positive feedback loop to regulate cell proliferation independent of telomerase activity. Nucleic Acids Res 50:3764-3776 (2022). PubMed: 35323972
  • Fu J  et al. MicroRNA-451a prevents cutaneous squamous cell carcinoma progression via the 3-phosphoinositide-dependent protein kinase-1-mediated PI3K/AKT signaling pathway. Exp Ther Med 21:116 (2021). PubMed: 33335579
  • Guarnaccia AD  et al. Impact of WIN site inhibitor on the WDR5 interactome. Cell Rep 34:108636 (2021). PubMed: 33472061
View all Publications for this product

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