Anti-PDPK1 抗体 (ab186870)
Key features and details
- Rabbit polyclonal to PDPK1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PDPK1 antibody
PDPK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDPK1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human PDPK1 aa 277-556 (C terminal).
Database link: O15530 -
ポジティブ･コントロール
- LOVO and mouse lung extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186870の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 63 kDa.
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| IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/2000. Predicted molecular weight: 63 kDa. |
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IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. -
組織特異性
Appears to be expressed ubiquitously. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. -
細胞内局在
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5170 Human
- Entrez Gene: 18607 Mouse
- Omim: 605213 Human
- SwissProt: O15530 Human
- SwissProt: Q9Z2A0 Mouse
- Unigene: 459691 Human
- Unigene: 10504 Mouse
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別名
- 3 phosphoinositide dependent protein kinase 1 antibody
- 3-phosphoinositide-dependent protein kinase 1 antibody
- hPDK 1 antibody
see all
画像
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Western blot - Anti-PDPK1 antibody (ab186870)All lanes : Anti-PDPK1 antibody (ab186870) at 1/500 dilution
Lane 1 : LOVO extract
Lane 2 : Mouse lung extract
Predicted band size: 63 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody (ab186870)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling PDPK1 with ab186870 at 1/200 dilution. Heat-mediated antigen retrieval with Tris-EDTA pH 9.0 for 15 minutes, 95-100oC.
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Immunocytochemistry/ Immunofluorescence - Anti-PDPK1 antibody (ab186870)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186870. Blue DAPI for nuclear staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody (ab186870)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue sections labeling PDPK1 with ab186870 at 1/200 dilution. Heat-mediated antigen retrieval with Tris-EDTA pH 9.0 for 15 minutes, 95-100oC.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab186870 は 3 報の論文で使用されています。
- You L et al. SDC2 Stabilization by USP14 Promotes Gastric Cancer Progression through Co-option of PDK1. Int J Biol Sci 19:3483-3498 (2023). PubMed: 37496999
- Wang X et al. PSMG2-controlled proteasome-autophagy balance mediates the tolerance for MEK-targeted therapy in triple-negative breast cancer. Cell Rep Med 3:100741 (2022). PubMed: 36099919
- Kim H et al. PDK1 is a negative regulator of axon regeneration. Mol Brain 14:31 (2021). PubMed: 33579325