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Anti-PDLIM7 抗体 (ab86065)

Submit a review Submit a question References (1)
Western blot - Anti-PDLIM7 antibody (ab86065)
  • Immunoprecipitation - Anti-PDLIM7 antibody (ab86065)

Key features and details

  • Rabbit polyclonal to PDLIM7
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-PDLIM7 antibody
    PDLIM7 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to PDLIM7

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Horse, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Bat

  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 125 and 175 of Human PDLIM7 (NP_005442.2)

  • ポジティブ･コントロール

    • Hela cell lysate

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab86065の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
IP
Use at 2-5 µg/mg of lysate.
特記事項
WB
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
IP
Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能

    May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway.

  • 組織特異性

    Isoform 1 and isoform 2 are expressed ubiquitously, however, isoform 2 predominates in skeletal muscle, isoform 1 is more abundant in lung, spleen, leukocytes and fetal liver.

  • 配列類似性

    Contains 3 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.

  • ドメイン

    The LIM zinc-binding 2 (LIM 2) domain interacts with TBX4.
    The LIM zinc-binding 3 (LIM 3) domain provides the structural basis for recognition of tyrosine-containing tight turn structures. This domain is necessary and sufficient for interaction with TBX5.
    Anchored to cell periphery via its N-terminal PDZ domain.

  • 細胞内局在

    Cytoplasm. Cytoplasm > cytoskeleton. Colocalizes with RET to the cell periphery and in some cytoskeletal components. Colocalizes with TPM2 near the Z line in muscle. Co-localizes with TBX4 and TBX5 to actin filaments.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 1110003B01Rik antibody
    • Enigma antibody
    • LIM domain protein antibody
    • LIM domain protein enigma antibody
    • LIM mineralization protein 1 antibody
    • Lim mineralization protein 3 antibody
    • LIM mineralization protein antibody
    • LMP 1 antibody
    • LMP antibody
    • LMP1 antibody
    • LMP3 antibody
    • PDLI7_HUMAN antibody
    • PDLIM 7 antibody
    • Pdlim7 antibody
    • PDZ and LIM doamin protein 7 antibody
    • PDZ and LIM domain 7 antibody
    • PDZ and LIM domain protein 7 antibody
    • Protein enigma antibody
    see all

画像

  • Western blot - Anti-PDLIM7 antibody (ab86065)
    Western blot - Anti-PDLIM7 antibody (ab86065)
    All lanes : Anti-PDLIM7 antibody (ab86065) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 50 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-PDLIM7 antibody (ab86065)
    Immunoprecipitation - Anti-PDLIM7 antibody (ab86065)
    Detection of PDLIM7 by Western blot of Immunprecipitate.
    ab86065, at 1 µg/ml, staining PDLIM7 in HeLa whole cell lysate immunoprecipitated using ab86065 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 3 seconds.

参考文献 (1)

ab86065 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab86065 は 1 報の論文で使用されています。

  • Ajeian JN  et al. Proteomic analysis of integrin-associated complexes from mesenchymal stem cells. Proteomics Clin Appl 10:51-7 (2016). PubMed: 26147903

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