AB86065

Anti-PDLIM7 抗体

Name: Anti-PDLIM7 抗体 (ab86065) | Abcam
Brand: Abcam Limited
SKU: AB86065
Price: 76000 JPY
Availability: InStock

Anti-PDLIM7 antibody

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(1 Publication)

Rabbit Polyclonal PDLIM7 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PDLIM7 aa 100-200.

別名を表示する

ENIGMA, PDLIM7, PDZ and LIM domain protein 7, LIM mineralization protein, Protein enigma, LMP

2 Images

Unknown

Immunoprecipitation - Anti-PDLIM7 antibody (AB86065)

Detection of PDLIM7 by Western blot of Immunprecipitate.
ab86065, at 1 µg/ml, staining PDLIM7 in HeLa whole cell lysate immunoprecipitated using ab86065 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-PDLIM7 antibody (ab86065)

Predicted band size: 50 kDa

false

Unknown

Western blot - Anti-PDLIM7 antibody (AB86065)

All lanes:

Western blot - Anti-PDLIM7 antibody (ab86065) at 0.04 µg/mL

Lane 1:

Hela whole cell lysate at 50 µg

Lane 2:

Hela whole cell lysate at 15 µg

Lane 3:

Hela whole cell lysate at 5 µg

Predicted band size: 50 kDa

true

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human PDLIM7 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NR12

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PDLIM7 also known as Enigma or LMP-1 is a protein involved in several cellular processes. It has a molecular mass of approximately 54 kDa and contains PDZ and LIM domains which facilitate its function. PDLIM7 is broadly expressed in tissues including heart skeletal muscle and osteoblasts. The protein acts mechanically by organizing cytoskeletal components and bridging interactions between actin filaments and other cellular structures.

Biological function summary

PDLIM7 plays a role in assembling protein complexes that influence structural and signaling pathways. This protein participates in bone development and remodeling processes linking it to skeletal system maintenance and repair. Through its LIM domains PDLIM7 facilitates the interaction with various binding partners. It serves as a scaffold helping to maintain proper cellular architecture and function.

Pathways

PDLIM7 integrates into the TGF-beta signaling cascade an important regulatory pathway in cellular differentiation and proliferation. It interacts with proteins such as SMADs which transmit signals from the cell membrane to the nucleus modulating gene expression. PDLIM7 also associates with the MAPK pathway influencing cellular responses to extracellular stimuli and stress.

Aberrant expression or function of PDLIM7 has been linked to bone-related diseases such as osteosarcoma and osteoporosis. Changes in PDLIM7 expression may disrupt normal bone density and growth contributing to these conditions. PDLIM7's interaction with proteins like SMADs in pathological contexts highlights its role in disease progression especially in diseases linked to abnormal bone formation or degradation.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway (By similarity).

See full target information PDLIM7

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Proteomics. Clinical applications 10:51-7 PubMed26147903

2015

Proteomic analysis of integrin-associated complexes from mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jila N Ajeian,Edward R Horton,Pablo Astudillo,Adam Byron,Janet A Askari,Angélique Millon-Frémillon,David Knight,Susan J Kimber,Martin J Humphries,Jonathan D Humphries

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDLIM7 抗体

Anti-PDLIM7 antibody

Immunoprecipitation - Anti-PDLIM7 antibody (AB86065)

Western blot - Anti-PDLIM7 antibody (AB86065)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise