Anti-PDLIM5/ENH 抗体 (ab85967)
Key features and details
- Rabbit polyclonal to PDLIM5/ENH
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PDLIM5/ENH antibody
PDLIM5/ENH 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDLIM5/ENH -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide corresponding to Human PDLIM5/ENH aa 500-600 (C terminal).
Database link: Q96HC4 -
ポジティブ･コントロール
- WB: HeLa whole cell lysate. IP: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85967の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 64 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses. -
組織特異性
Heart and skeletal muscle specific. Expression is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression. -
配列類似性
Contains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain. -
細胞内局在
Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse > synaptosome. Cytoplasm. Detected both at presynaptic and postsynaptic sites. - Information by UniProt
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参照データベース
- Entrez Gene: 503621 Cow
- Entrez Gene: 10611 Human
- Entrez Gene: 56376 Mouse
- Entrez Gene: 64353 Rat
- Omim: 605904 Human
- SwissProt: Q96HC4 Human
- SwissProt: Q8CI51 Mouse
- SwissProt: Q62920 Rat
see all -
別名
- ENH antibody
- ENH1 antibody
- Enigma homolog antibody
see all
画像
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Western blot - Anti-PDLIM5/ENH antibody (ab85967)All lanes : Anti-PDLIM5/ENH antibody (ab85967) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 64 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-PDLIM5/ENH antibody (ab85967)1mg HeLa whole cell lysate was immunoprecipitated using ab85967 at 3µg. 20% of IP was loaded/lane and PDLIM5/ENH was detected with ab85967 at 1µg/ml (lane 1) or a control IgG (lane 2).
Detection: chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab85967 は論文での使用が確認できていません。