Anti-PDLIM2 抗体 (ab68220)
Key features and details
- Mouse polyclonal to PDLIM2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PDLIM2 antibody
PDLIM2 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to PDLIM2 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human PDLIM2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- PDLIM2 transfected 293T cell lysate and human liver tissue lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68220の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 36, 38 kDa (predicted molecular weight: 37 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 36, 38 kDa (predicted molecular weight: 37 kDa). |
ターゲット情報
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機能
Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity. -
配列類似性
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
細胞内局在
Cytoplasm > cytoskeleton; Nucleus; Cytoplasm. Nucleus. May be partially nuclear and Cytoplasm > cytoskeleton. Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin. - Information by UniProt
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参照データベース
- Entrez Gene: 64236 Human
- Omim: 609722 Human
- SwissProt: Q96JY6 Human
- Unigene: 632034 Human
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別名
- FLJ34715 antibody
- MYSTIQUE antibody
- OTTHUMP00000162522 antibody
see all
画像
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Western blot - Anti-PDLIM2 antibody (ab68220)Anti-PDLIM2 antibody (ab68220) at 1/500 dilution + human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 37 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
Western blot - Anti-PDLIM2 antibody (ab68220)All lanes : Anti-PDLIM2 antibody (ab68220) at 1/500 dilution
Lane 1 : PDLIM2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (4)
ab68220 は 4 報の論文で使用されています。
- Li Z et al. Central Nervous System-Infecting Pathogens Escherichia coli and Cryptococcus neoformans Exploit the Host Pdlim2 for Intracellular Traversal and Exocytosis in the Blood-Brain Barrier. Infect Immun 89:e0012821 (2021). PubMed: 34228504
- Jin M et al. Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin promotes inflammation in mouse testes: The critical role of Klotho in Sertoli cells. Toxicol Lett 295:134-143 (2018). WB . PubMed: 29885354
- Wurster KD et al. Inactivation of the putative ubiquitin-E3 ligase PDLIM2 in classical Hodgkin and anaplastic large cell lymphoma. Leukemia 31:602-613 (2017). WB ; Human . PubMed: 27538486
- Vanoirbeek E et al. PDLIM2 expression is driven by vitamin D and is involved in the pro-adhesion, and anti-migration and -invasion activity of vitamin D. Oncogene N/A:N/A (2013). Human . PubMed: 23584482
