AB4644

Anti-PDI1 抗体 [38H8]

Anti-PDI1 antibody [38H8]

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(5 Publications)

Mouse Monoclonal PDI antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 5 publications.

別名を表示する

MFP1, Pdi1p, Protein disulfide-isomerase, TRG1, Thioredoxin related glycoprotein 1

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

38H8

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

交差種

Saccharomyces cerevisiae

アプリケーション

ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Suggested working dilution of 1/5000 (see PMID: 17051412).</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500 - 1/5000", "ICCIF-species-notes": "<p>(gives an ER staining pattern on yeast cells).</p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

バッファー組成

Preservative: 0.065% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Protein Disulfide Isomerase 1 (PDI1) also known as PDIA1 is a multifunctional enzyme that facilitates protein folding and thiol-disulfide exchange reactions within the endoplasmic reticulum (ER). It has a molecular weight of approximately 57 kDa. PDI1 is expressed abundantly in the endoplasmic reticulum of various cell types especially in tissues like the liver where it aids in processing secretory and membrane proteins. The protein's thioredoxin-like domains play an important role in oxidizing or reducing disulfide bonds ensuring proper protein conformation.

Biological function summary

PDI1 acts beyond conventional disulfide bond formation and isomerization. It serves as a chaperone that prevents protein aggregation during oxidative protein folding. PDI1 is part of the protein disulfide isomerase family which interacts in a complex network to maintain protein homeostasis. It works together with ERp57 and other members to assist in the quality control of nascent proteins ensuring functional and correctly folded proteins are distributed within the cell.

Pathways

PDI1 is integral to the unfolded protein response (UPR) and protein folding pathway within the ER. During cellular stress PDI1 and other enzymes like BiP manage misfolded proteins preventing accumulation that could lead to ER stress. PDI1 also interacts with the calnexin/calreticulin cycle facilitating proper assembly of glycoproteins in conjunction with ERp72 ensuring that cellular pathways dependent on ER function proceed smoothly.

PDI1 is implicated in neurodegenerative disorders like Alzheimer's disease and cardiovascular diseases such as atherosclerosis. In Alzheimer's disease PDI1's impaired function may lead to the accumulation of misfolded proteins including amyloid beta. It interacts with other ER stress-related proteins like GRP78 in managing stress responses. In cardiovascular diseases PDI1's role in maintaining redox balance and protein folding can affect vascular health and integrity when disrupted and it works alongside proteins like atherogenic lipoproteins.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information PDI1

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:102533 PubMed36162502

2022

Eisosome protein Pil1 regulates mitochondrial morphology, mitophagy, and cell death in Saccharomyces cerevisiae.

Applications

Unspecified application

Species

Unspecified reactive species

Amita Pal,Arun Kumar Paripati,Pallavi Deolal,Arpan Chatterjee,Pushpa Rani Prasad,Priyanka Adla,Naresh Babu V Sepuri

Cell death & disease 11:832 PubMed33028809

2020

LncRNA LL22NC03-N14H11.1 promoted hepatocellular carcinoma progression through activating MAPK pathway to induce mitochondrial fission.

Applications

Unspecified application

Species

Unspecified reactive species

Tingzhuang Yi,Hongcheng Luo,Fengxue Qin,Qi Jiang,Shougao He,Tonghua Wang,Jianwei Su,Sien Song,Xiaoshan Qin,Yueqiu Qin,Xihan Zhou,Zansong Huang

Nature communications 11:781 PubMed32034123

2020

De novo emergence of adaptive membrane proteins from thymine-rich genomic sequences.

Applications

Unspecified application

Species

Unspecified reactive species

Nikolaos Vakirlis,Omer Acar,Brian Hsu,Nelson Castilho Coelho,S Branden Van Oss,Aaron Wacholder,Kate Medetgul-Ernar,Ray W Bowman,Cameron P Hines,John Iannotta,Saurin Bipin Parikh,Aoife McLysaght,Carlos J Camacho,Allyson F O'Donnell,Trey Ideker,Anne-Ruxandra Carvunis

Free radical biology & medicine 52:2000-12 PubMed22406321

2012

Oxidative protein folding and unfolded protein response elicit differing redox regulation in endoplasmic reticulum and cytosol of yeast.

Applications

Species

Unspecified reactive species

Marizela Delic,Corinna Rebnegger,Franziska Wanka,Verena Puxbaum,Christina Haberhauer-Troyer,Stephan Hann,Gunda Köllensperger,Diethard Mattanovich,Brigitte Gasser

Applied microbiology and biotechnology 74:381-9 PubMed17051412

2006

Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris.

Applications

Unspecified application

Species

Unspecified reactive species

Leonardo M Damasceno,Kyle A Anderson,Gerd Ritter,James M Cregg,Lloyd J Old,Carl A Batt

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDI1 抗体 [38H8]

Anti-PDI1 antibody [38H8]

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

Reactivity data

出荷温度及び保存条件

製品の状態

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

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Secondary Antibodies

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Primary Antibodies

Abcam product promise