Anti-PDGF BB 抗体 (ab9704)

The lab let me know that this antibody (ab84265, detection antibody) can be used with ab9704 as capture antibody and ab9706 as protein standard.

Thank you for contacting Abcam.
Earlier we discussed the specificity of ab9704 for detecting PDGF BB as well as the expected expression of PDGF BB across cell lines and tissues.
According to the human protein atlas, PDGF B (alternative name for PDGF BB), is seen across many different cell lines and in a variety of tissues. Specifically, it appears that lung macrophages have moderate - strong IHC staining for PDGF B in their cytoplasm. Below is a link to the protein atlas information:
http://www.proteinatlas.org/ENSG00000100311/normal
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thank you for contacting us, I apologize for the delay in my reply. I would recommend ab114130 for your purposes. The immunogen for this antibody shows no significant similarity to PDGFA, and it is slightly less likely to react with mouse than our other rabbit polyclonal antibodies. That being said, the mouse protein is fairly homologous with human and there is still a chance that ab114130 may show some reactivity to mouse PGDF BB.
If you do want to use the mouse monoclonal antibody ab51869 on mouse tissue, you may need to perform an additional blocking step to prevent mouse-on-mouse background staining. If mouse-on-mouse background is a problem in your assay, it will be visible when running your no primary antibody or isotype control. To reduce this background, you can perform an additional blocking step after your normal serum block and before the primary antibody incubation. The sections should be incubated for 1 hour with a F(ab) fragment unconjugated secondary antibody to mouse IgG, such as ab6668, at a ˜1:100 dilution at room temperature.
I hope this helps, please let me know if you need any additional information or assistance.

I have pasted the supplier's protocol below, but please do not hesitate to contact us should you have any other questions. Neutralization Assay for Anti-h-PDGF-BB using 3T3 cells Antibody: Anti-Human PDGF Procedure: 1) Resuspend 3T3 cells in regular culture media with 10% calf serum (Gibco lot# 1023609). Transfer 100ml culture/well to assay plate (5,000 cells/well). Incubate overnight. 2) Complete media change. Replace with 100ml assay media containing 0.5% calf serum. Incubate overnight. 3) Complete media change with assay media. 4) Serial dilute a-h-PDGF in assay media in triplicate. 5) Transfer assay media containing 8ng/ml h-PDGF to serial diluted antibody. Volume/well is 120 ml. Incubate 1 hour. 6) Transfer 100ml h-PDGF/anti-h-PDGFcomplex to cells in assay plate. Volume of each well is 200ml,containing 2 ng/ml h-PDGF and anti-h-PDGFas indicated below. Incubate 46 hours. 7) Add 20ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well. 8) Incubate 37oC and read at OD 490 nm.

Unfortunately, we do not have it in stock. However, from placing your order it will take approximately 2 working days for us to obtain this antibody, followed by another 24 hrs to ship the product to you.