Anti-PDGF B 抗体 [MM0014-5F66] (ab51869)
![Western blot - Anti-PDGF B antibody [MM0014-5F66] (ab51869)](https://www.abcam.co.jp/ps/products/51/ab51869/Images/ab51869-471133-anti-pdgf-b-antibody-mm0014-5f66-western-blot-placenta-human.jpg "Western blot - Anti-PDGF B antibody [MM0014-5F66] (ab51869)")
Key features and details
- Mouse monoclonal [MM0014-5F66] to PDGF B
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-PDGF B antibody [MM0014-5F66]
PDGF B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MM0014-5F66] to PDGF B -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein (Human)
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ポジティブ･コントロール
- WB: Human placenta tissue lysate. IHC-P: Human placenta tissue. Flow Cyt: SHSY-5Y cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
MM0014-5F66 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51869の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 27 kDa.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-P |
1/50 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000. Predicted molecular weight: 27 kDa. |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P
1/50 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 5155 Human
- Omim: 190040 Human
- SwissProt: P01127 Human
- Unigene: 1976 Human
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別名
- Becaplermin antibody
- c sis antibody
- FLJ12858 antibody
see all
画像
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Western blot - Anti-PDGF B antibody [MM0014-5F66] (ab51869)Anti-PDGF B antibody [MM0014-5F66] (ab51869) at 1/1000 dilution + Human placenta tissue lysate
Predicted band size: 27 kDa
Observed band size: 27 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody [MM0014-5F66] (ab51869)](https://www.abcam.co.jp/ps/products/51/ab51869/Images/ab51869-63939-anti-pdgf-bb-antibody-mm0014-5f66-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF BB antibody [MM0014-5F66] (ab51869)ab51869 at 1/200 dilution, staining PDGF BB in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Antigen retrieval was done using proteinase K (20 µg/mL in PBS: Room temperature, ranging from 5 min to 30 mins).
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![Flow Cytometry - Anti-PDGF BB antibody [MM0014-5F66] (ab51869)](https://www.abcam.co.jp/ps/products/51/ab51869/Images/ab51869-63938-anti-pdgf-bb-antibody-mm0014-5f66-flow-cytometry.jpg)
Flow Cytometry - Anti-PDGF BB antibody [MM0014-5F66] (ab51869)Overlay histogram showing SHSY-5Y cells stained with ab51869 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51869, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab51869 は 6 報の論文で使用されています。
- Li Y et al. Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer. Theranostics 12:3818-3833 (2022). PubMed: 35664061
- Xie G et al. Conjugated secondary 12a-hydroxylated bile acids promote liver fibrogenesis. EBioMedicine 66:103290 (2021). PubMed: 33752128
- Silva CR et al. Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration. Acta Biomater 77:155-171 (2018). PubMed: 30031163
- Miyata T et al. The roles of platelet-derived growth factors and their receptors in brain radiation necrosis. Radiat Oncol 9:51 (2014). IHC-P, ICC/IF ; Human . PubMed: 24512807
- Niu F et al. Tat 101-mediated enhancement of brain pericyte migration involves platelet-derived growth factor subunit B homodimer: implications for human immunodeficiency virus-associated neurocognitive disorders. J Neurosci 34:11812-25 (2014). IHC-P, ICC/IF . PubMed: 25164676
- Suzuki S et al. Clinicopathological significance of platelet-derived growth factor (PDGF)-B and vascular endothelial growth factor-A expression, PDGF receptor-ß phosphorylation, and microvessel density in gastric cancer. BMC Cancer 10:659 (2010). WB ; Human . PubMed: 21118571
