Anti-PDE7A/HCP1 抗体 [EPR7731] (ab154857)
![Western blot - Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857)](https://www.abcam.co.jp/ps/products/154/ab154857/Images/ab154857-161484-anti-pde7a-antibody-epr7731-western-blot.jpg "Western blot - Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7731] to PDE7A/HCP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PDE7A/HCP1 antibody [EPR7731]
PDE7A/HCP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7731] to PDE7A/HCP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human PDE7A/HCP1.
Database link: Q13946 -
ポジティブ･コントロール
- HuT78 cell lysate; Human skeletal muscle tissue; HuT78 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7731 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154857の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| ICC/IF |
1/250 - 1/500.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
追加情報
Is unsuitable for Flow Cyt or IP.
ターゲット情報
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機能
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction. -
組織特異性
PDE7A1 is found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. PDE7A2 is found abundantly in skeletal muscle and at low levels in heart. -
パスウェイ
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily. -
発生段階
Developmentally regulated. PDE7A1 and PDE7A2 are found in several fetal tissues, expression is reduced throughout development. It persists strongly only in adult skeletal muscle. -
ドメイン
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain. -
細胞内局在
Cytoplasm > cytosol. PDE7A1 (57 kDa) is located mostly to soluble cellular fractions and Cytoplasm. PDE7A2 (50 kDa) is located to particulate cellular fractions. - Information by UniProt
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参照データベース
- Entrez Gene: 5150 Human
- Omim: 171885 Human
- SwissProt: Q13946 Human
- Unigene: 527119 Human
- Unigene: 728847 Human
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別名
- 5''-cyclic phosphodiesterase 7A antibody
- HCP 1 antibody
- HCP1 antibody
see all
画像
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Western blot - Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857)Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857) at 1/1000 dilution + HuT78 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 56 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857)](https://www.abcam.co.jp/ps/products/154/ab154857/Images/ab154857-161483-anti-pde7a-antibody-epr7731-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PDE7A/HCP1 with ab154857 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857)](https://www.abcam.co.jp/ps/products/154/ab154857/Images/ab154857-161482-anti-pde7a-antibody-epr7731-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857)Immunofluorescent analysis of HuT78 cells labeling PDE7A/HCP1 with ab154857 at 1/250 dilution.
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![Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857)](https://www.abcam.co.jp/ps/products/154/ab154857/Images/ab154857-4-benefits-of-recombinant-antibodies.png)
Anti-PDE7A/HCP1 antibody [EPR7731] (ab154857)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab154857 は 3 報の論文で使用されています。
- Cai Y et al. Genomic Alterations in PIK3CA-Mutated Breast Cancer Result in mTORC1 Activation and Limit the Sensitivity to PI3Kα Inhibitors. Cancer Res 81:2470-2480 (2021). PubMed: 33685991
- Li EA et al. Phosphodiesterase expression in the normal and failing heart. Arch Biochem Biophys 662:160-168 (2019). PubMed: 30550727
- Yamamoto N et al. The tumor-suppressive microRNA-1/133a cluster targets PDE7A and inhibits cancer cell migration and invasion in endometrial cancer. Int J Oncol 47:325-34 (2015). PubMed: 25955017