Anti-PDE5A/PDE5 抗体 (ab28761)
Key features and details
- Goat polyclonal to PDE5A/PDE5
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-PDE5A/PDE5 antibody
PDE5A/PDE5 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PDE5A/PDE5 -
由来種
Goat -
特異性
This antibody is expected to recognise all 4 reported isoforms (as represented by NP_001074; NP_236914; NP_246273). [NP_237223 has been retracted]. -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow, Dog
-
免疫原
Synthetic peptide corresponding to Human PDE5A/PDE5 aa 864-875 (C terminal).
Sequence:C-LINGESG QAKRN
(Peptide available asab45684) -
ポジティブ･コントロール
- Human lung lysate. Lysate prepared from transfected HEK293 cells transiently expressing PDE5A/PDE5 Flow Cyt (Intra): HEK293 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28761の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
|
| WB | (1) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
1 hour primary incubation is recommended for this product. |
| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). 1 hour primary incubation is recommended for this product. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
-
機能
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. -
組織特異性
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung. -
パスウェイ
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. -
配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
ドメイン
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B. -
翻訳後修飾
Phosphorylation is regulated by binding of cGMP to the two allosteric sites. - Information by UniProt
-
参照データベース
- Entrez Gene: 281972 Cow
- Entrez Gene: 403825 Dog
- Entrez Gene: 8654 Human
- Entrez Gene: 171115 Rat
- Omim: 603310 Human
- SwissProt: Q28156 Cow
- SwissProt: O77746 Dog
- SwissProt: O76074 Human
see all -
別名
- 5''-cyclic phosphodiesterase antibody
- CGB PDE antibody
- CGB-PDE antibody
see all
画像
-
Western blot - Anti-PDE5A/PDE5 antibody (ab28761)Anti-PDE5A/PDE5 antibody (ab28761) at 1 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing PDE5A/PDE5 (isoform 2)
Predicted band size: 100 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
Flow Cytometry (Intracellular) - Anti-PDE5A/PDE5 antibody (ab28761)Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab28761 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
-
Immunocytochemistry/ Immunofluorescence - Anti-PDE5A/PDE5 antibody (ab28761)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab28761 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
データシートおよび資料
-
Datasheet download
参考文献 (2)
ab28761 は 2 報の論文で使用されています。
- Xiang T et al. The novel ZEB1-upregulated protein PRTG induced by Helicobacter pylori infection promotes gastric carcinogenesis through the cGMP/PKG signaling pathway. Cell Death Dis 12:150 (2021). PubMed: 33542225
- Wang J et al. High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells. Front Oncol 11:716246 (2021). PubMed: 34745942
