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AB99409

Anti-PDE4D 抗体

Anti-PDE4D antibody

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(2 Publications)

Rabbit Polyclonal PDE4D antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PDE4D aa 700-800.

別名を表示する

DPDE3, PDE4D, PDE43, cAMP-specific phosphodiesterase 4D

2 Images
Immunoprecipitation - Anti-PDE4D antibody (AB99409)
  • IP

Unknown

Immunoprecipitation - Anti-PDE4D antibody (AB99409)

ab99409 at 1µg/ml detecting PDE4D in HeLa whole cell lysate by WB following IP.

Lane 1 : ab99409 at 3µg/mg of lysate
Lane 2 : IP with an antibody which recognizes an downstream epitope of PDE4D
Lane 3 : control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PDE4D antibody (ab99409)

Predicted band size: 91 kDa

false

Western blot - Anti-PDE4D antibody (AB99409)
  • WB

Unknown

Western blot - Anti-PDE4D antibody (AB99409)

All lanes:

Western blot - Anti-PDE4D antibody (ab99409) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 91 kDa

true

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human PDE4D aa 700-800. The exact immunogen used to generate this antibody is proprietary information.

Q08499

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 4D (PDE4D) is an enzyme that hydrolyzes cyclic AMP (cAMP) to its inactive form AMP controlling important cellular signaling pathways. It is part of the large PDE family and sometimes referred to as DPDE1. With a molecular weight of approximately 93 kDa PDE4D is widely expressed in the brain heart lungs and immune cells. Its expression in these tissues indicates an important regulatory role in both central and peripheral physiological processes.
Biological function summary

PDE4D plays an important role in the modulation of intracellular cAMP levels impacting processes such as inflammation cognition and cardiac function. It does not operate as part of a larger complex but collaborates with other signaling molecules to influence cellular responses. PDE4D's regulation of cAMP levels directly affects protein kinase A (PKA) leading to alterations in gene expression and cell activity.

Pathways

PDE4D integrates into the cAMP signaling and inflammatory pathways impacting various cellular responses. In the cAMP signaling pathway it partners with other PDEs and signaling proteins such as PKA which phosphorylates target proteins affecting cellular function. In inflammatory pathways PDE4D modulates immune cell activity by altering the cAMP-mediated inhibition of pro-inflammatory cytokine production linking it to inflammation-related responses.

PDE4D has associations with conditions such as asthma and ischemic stroke. In asthma PDE4D regulates cAMP levels in lung cells affecting bronchial constriction and inflammation. In ischemic stroke alterations in PDE4D activity influence vascular function by changing cAMP dynamics in vascular smooth muscle cells. Within these disorders PDE4D interacts with proteins like cytokines and COX-2 which also play parts in inflammatory pathways and vascular responses.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
See full target information PDE4D
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2023:6623329 PubMed37501933

2023

Vitronectin Destroyed Intestinal Epithelial Cell Differentiation through Activation of PDE4-Mediated Ferroptosis in Inflammatory Bowel Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxu Pan,Li Xiang,Xinhua Liang,Wenjun Du,Junhong Zhao,Song Zhang,Xuan Zhou,Lanlan Geng,Sitang Gong,Wanfu Xu

EMBO reports 23:e54128 PubMed35876654

2022

LINC00839 promotes colorectal cancer progression by recruiting RUVBL1/Tip60 complexes to activate NRF1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoting Liu,Jianxiong Chen,Sijing Zhang,Xunhua Liu,Xiaoli Long,Jiawen Lan,Miao Zhou,Lin Zheng,Jun Zhou
View all publications
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代替製品

Primary Antibodies

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Anti-PDE4D (phospho S190) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4D (phospho S190) antibody (AB59212)

  • 0
  • 1
Primary Antibodies

AB14613

Anti-PDE4D antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4D antibody (AB14613)

  • 4
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Primary Antibodies

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Anti-PDE4D antibody [EPR11823-29]

RabMAb|Recombinant|Trial Size

Western blot - Anti-PDE4D antibody [EPR11823-29] (AB171749)

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  • 0
Primary Antibodies

AB171750

Anti-PDE4D antibody [EPR11823-31]

RabMAb|Recombinant

Western blot - Anti-PDE4D antibody [EPR11823-31] (AB171750)

  • 4
  • 1

組合せ製品

Primary Antibodies

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  • 0
  • 0
Secondary Antibodies

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  • 5
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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
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