Anti-PDE4D 抗体 (ab14613)

Thank you for your reply and for your understanding in this matter.

As requested, I am sending ab14628 as a free of charge replacement for you and you should have it on Tuesday morning. For your records the new invoice number is *.

Also, as a thank you for your patience, below is a discount code that will give 25% off 1 item on your next order with Abcam:

Discount Code: *
Exp Date: *

If there is anything else I can help you with, please let me know.

I have tried 3 times to contact the supplier to ask them to provide those images, but they are not replying to my emails. I do apologize for the inconvenience this is causing you and I am grateful for your patience in the matter.

I believe the best way forward is to replace or refund the cost of the antibody for you. Please let me know how you would like to proceed and I will be happy to bring about a resolution to this issue.

I look forward to your reply.

Thank you for your patience in this matter.

I have heard back from the supplier in regards to ab14613 and they have tried to go back and look for old data on PDE4D antibody. They could not find the blot on HEK cells. They do have blots on other cells lines including SHS5Y neuroblastoma, rat primary neuronal cultures and in cells over expressing various PDE4D subtypes but not HEK293 cells. Those images were not included in the email they sent me.

I am very sorry about this matter and I would be happy to either replace or refund the current vial of ab14613 that you have.

Please let me know how you would like to proceed in this matter.

Thank you for contacting Abcam earlier today.

I am sorry about the western blot issues you have been having with ab14613. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

I have talked to the lab about this and they have told me one of the reason there is a band at 30kDa may be proteolytic degradation. We have checked this antibody on HEK cells and it labels 3 different PDE4D variants. Do you include protease cocktail during sample preparation? If not, I would suggest that you repeat the lysis and include the protease inhibitors.

If you already do include protease inhibitors, then I think there may be an issue with the antibody and the best way forward is to send you ab87239 as a free of charge replacement (as we discussed on the telephone).

I look forward to your reply and helping to resolve this issue.

Thank you for contacting us. I apologise for the delay, I wanted to check this case with my lab colleagues.
Regarding ab59249, the amino acid range of this immunogen is aaXXXX of human PDE4D with accession# Q08499 (http://www.uniprot.org/uniprot/Q08499).
This antibody binds to isoforms 4, 3, 5, N3, 8, 9, 7, and 12. It does not bind to isoform 10, 1, 2, and 6. According to the isoforms that this antibody binds, you would expect to see two bands, approximately 25 and 85 kDa. Because the western blot on our website is cut off at 34 kDa, we can retest this antibody to see if it does actually bind the lower isoform as well.
Although we haven’t specifically tested the antibody against all isoforms, there are other alternatives that may also recognise several forms of the protein:
For ab77080 we ran a BLAST search and found the antigen sequence in all of 9 isoforms:
https://www.abcam.com/PDE4D-antibody-ab77080.html
Ab112018 also shares 100% identity to isoforms PDE4D1-9:
https://www.abcam.com/PDE4D-antibody-ab112018.html
Ab99409 and ab87329 recognize 10 of 12 isoforms listed for SwissProt entry Q08499. The two isoforms listed for SwissProt Q08499 not recognized by either of them are sp|Q08499-7|PDE4D_HUMAN and sp|Q08499-12|PDE4D_HUMAN:
http://www.uniprot.org/uniprot/Q08499
And ab87329’s immunogen is present in 8 out of 12 of them so it should detect isoforms 1- 10.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements for ab14611 and ab14613with the order number 1137676.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for contacting Abcam.

Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Thank you for contacting us and sorry for the delay in response. I now have confirmation of the specificity of the two antibodies in question. ab14613 has been raised against a C-terminal peptide sequence of PDE4D. This peptide, contrary to what was published on the datasheet should recognise all 9 isoforms. I am sorry for this misinformation. This is as a result of science moving on and new isoforms being identified. This will now be updated on the datasheet. Similarly, ab77080 has been raised against the C-terminal amino acids (QPEACVIDDRSPDT) of human PDE4D this should also detect all 9 isoforms of PDE4D. If you have any further questions please do not hesitate to ask.