JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB151454

Anti-PDE10A 抗体 - C-terminal

Anti-PDE10A antibody - C-terminal

4

(1 Review)

|

(3 Publications)

Rabbit Polyclonal PDE10A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDE10A aa 500 to C-terminus.

別名を表示する

PDE10A

6 Images
Western blot - Anti-PDE10A antibody - C-terminal (AB151454)
  • WB

Supplier Data

Western blot - Anti-PDE10A antibody - C-terminal (AB151454)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-PDE10A antibody - C-terminal (ab151454) at 1/1000 dilution

Lane 1:

H1299 whole cell extracts at 30 µg

Lane 2:

HCT116 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Western blot - Anti-PDE10A antibody - C-terminal (AB151454)
  • WB

Supplier Data

Western blot - Anti-PDE10A antibody - C-terminal (AB151454)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-PDE10A antibody - C-terminal (ab151454) at 1/1000 dilution

All lanes:

HCT116 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Western blot - Anti-PDE10A antibody - C-terminal (AB151454)
  • WB

Supplier Data

Western blot - Anti-PDE10A antibody - C-terminal (AB151454)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-PDE10A antibody - C-terminal (ab151454) at 1/10000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Western blot - Anti-PDE10A antibody - C-terminal (AB151454)
  • WB

Supplier Data

Western blot - Anti-PDE10A antibody - C-terminal (AB151454)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-PDE10A antibody - C-terminal (ab151454) at 1/3000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 88 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE10A antibody - C-terminal (AB151454)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE10A antibody - C-terminal (AB151454)

Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling PDE10A with ab151454 at 1/500.

Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody - C-terminal (AB151454)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody - C-terminal (AB151454)

Immunofluorescence analysis of methanol fixed HeLa cells labeling PDE10A with ab151454 at 1/200. The lower panel shows costaining with Hoechst 33342.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human PDE10A aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y233

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 10A (PDE10A) also known as phosphodiesterase 10 is an enzyme that hydrolyzes cyclic nucleotides such as cAMP and cGMP into non-cyclic forms which reduces their signaling effects. PDE10A has a molecular weight of approximately 89 kDa. This enzyme shows higher expression in the brain especially in the striatum a region important for motor control and certain cognitive functions. It occurs in other tissues at lower levels but its role there is less clear.
Biological function summary

PDE10A modulates intracellular signaling pathways critical for neurological processes by controlling levels of cyclic nucleotides. Although PDE10A acts independently it interacts with various signaling molecules affecting neuronal signaling. It exerts significant influence over the dopamine and glutamate neurotransmitter systems important for regulating neuronal excitability and synaptic transmission.

Pathways

Several neurological pathways rely on the function of PDE10A to maintain homeostasis. The enzyme plays a role in the cAMP and cGMP signaling pathways both of which are fundamental in mediating various cellular responses. PDE10A shares functional relationships with other phosphodiesterase family members such as PDE1 and PDE2 which also regulate cyclic nucleotide levels in the central nervous system.

An altered PDE10A function links to several neurological conditions including Huntington's disease and schizophrenia. Dysregulation of dopamine signaling within these disorders implicates PDE10A since it modulates this neurotransmitter pathway. In schizophrenia abnormal PDE10A activity could affect proteins like D2 dopamine receptors contributing to altered signaling in neural circuits associated with this disorder.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides (PubMed : 10373451, PubMed : 10393245, PubMed : 16330539, PubMed : 17389385, PubMed : 27058447). Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate (PubMed : 10373451, PubMed : 10393245, PubMed : 17389385, PubMed : 27058447). May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition (PubMed : 27058447).
See full target information PDE10A
style
left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Brain : a journal of neurology 148:1588-1603 PubMed39546628

2024

Single-nucleus transcriptomics reveals disease- and pathology-specific signatures in α-synucleinopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Gonzalo S Nido,Martina Castelli,Sepideh Mostafavi,Anna Rubiolo,Omnia Shadad,Guido Alves,Ole-Bjørn Tysnes,Irene H Flønes,Christian Dölle,Charalampos Tzoulis

The Prostate 82:97-106 PubMed34633095

2021

AT-rich interaction domain 5A regulates the transcription of interleukin-6 gene in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wataru Ikeuchi,Yuriko Wakita,Guoxiang Zhang,Chun Li,Keiichi Itakura,Takahiro Yamakawa

Molecular therapy : the journal of the American Society of Gene Therapy 29:1086-1101 PubMed33221436

2020

PDE10A Inhibition Reduces the Manifestation of Pathology in DMD Zebrafish and Represses the Genetic Modifier PITPNA.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias R Lambert,Janelle M Spinazzola,Jeffrey J Widrick,Anna Pakula,James R Conner,Janice E Chin,Jane M Owens,Louis M Kunkel
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB227829

Anti-PDE10A antibody [EPR22383]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (AB227829)

  • 0
  • 0
Primary Antibodies

AB245198

Anti-PDE10A antibody [EPR22383] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] - BSA and Azide free (AB245198)

  • 0
  • 0

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB236632

Anti-HA tag antibody [EPR22819-101]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (AB236632)

  • 3
  • 1
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com